GB/T 36851-2018 in English

VALID

Detection and identification of Xanthomonas vesicatoria

Issued on:2018-09-17
Implemented on:2019-04-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $220.00
$214.00

Standard No:	GB/T 36851-2018
Document status:	VALID
Title in English:	Detection and identification of Xanthomonas vesicatoria
Title in Chinese:	辣椒细菌性斑点病菌检疫鉴定方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-09-17
Implemented on:	2019-04-01
ICS Classification:	65.020.01-Farming and forestry in general
Chinese Classification:	B16-Plant quarantine, pest control
Professional Classification:	GB-National Standard
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《GB/T 36851-2018辣椒细菌性斑点病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

1. Background and significance of standard formulation

The formulation of the National Standard of the People's Republic of China GB/T 36851-2018 "Quarantine and Identification Methods for Pepper Bacterial Spot Disease" aims to standardize the quarantine and identification process of pepper bacterial spot disease (Xanthomonas vesicatoria) in pepper and its products, and ensure the safety of agricultural production and the smooth progress of international trade. This standard was proposed and managed by the National Technical Committee for Plant Quarantine and Standardization, and the main drafting units include the China Institute of Inspection and Quarantine, the Suifenhe Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, etc.

2. Basic information of bacterial spot of pepper

Classification information	Transmission route	Host range	Geographical distribution
Xanthomonas vesicatoria	Infected seeds, plant residues, rain splash	Pepper, tomato, various Solanaceae crops	Major agricultural areas in the world, including China
Bacterial leaf spot of pepper	- Long-distance transmission: infected seeds	- Contact transmission: seedling transplanting	Africa, America, Asia, Europe, Oceania

3. Interpretation of quarantine identification methods

Detection of Xanthomonas vesicatoria usually includes three parts: symptom inspection, isolation culture and molecular biological detection.

3.1 Symptom inspection and sample preparation

Preliminary judgment is made based on the symptoms of the host plant, focusing on the characteristics of the lesions on the leaves and fruits. Plants with typical symptoms need to be photographed and sent to the laboratory for testing.

3.2 Molecular biological detection

PCR gel electrophoresis or real-time fluorescence PCR technology is used for detection. Specific primers (such as Bs-XvF and Bs-XvR) were used in the PCR reaction system, and the size of the amplified product was 138 bp.

3.3 Isolation, culture and identification

The mTMB and CKTM selective culture media were used for isolation and culture, and the characteristics of the colonies included yellow and viscous. Combined with the Biolog system for automatic identification of microorganisms.

4. Implementation suggestions

Choose appropriate detection technology:Choose PCR or real-time fluorescence PCR method according to laboratory conditions, and ensure sufficient supply of reagents and equipment.
Optimize sample collection and preparation:Strictly follow the standard operating procedures (SOP) for plant and seed sample processing to avoid contamination.
Strengthen laboratory capacity building:Equip with necessary instruments and equipment (such as PCR instruments, real-time fluorescence PCR instruments, autoclaves, etc.), and conduct regular staff training.
Establish a data sharing mechanism:Unify the recording format of test results, promote data sharing and analysis, and improve quarantine efficiency across the country.

More samples: DL/T 368-2010 JJG 21-2008 GB 50021-2001(2009)

Sample only — not a preview of GB/T 36851-2018

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