GB/T 36875-2018 in English

Interpretation of the National Standard of the People's Republic of China GB/T 36875—2018

Overview of RT-nPCR Detection Method for Classical Swine Fever Virus

This standard specifies the technical requirements for the reverse transcription-nested polymerase chain reaction (RT-nPCR) method specific to classical swine fever virus, and is applicable to the detection of classical swine fever virus nucleic acid in organs, blood, feces and cell cultures of pigs that may be infected with classical swine fever virus.

Background and significance of standard formulation

Classic swine fever is a highly contagious animal disease that seriously endangers the global pig industry, and its diagnosis is crucial to controlling the spread of the epidemic. This standard is based on reverse transcription-nested PCR technology, combined with laboratory conditions and reagent preparation requirements to ensure the accuracy and repeatability of the detection method.

Reagent preparation and operation steps

Result determination and quality control

Observe the specific bands of the inner PCR product by electrophoresis, and the positive control should show a 272bp amplification band. Negative and positive controls should be set up in the experiment to ensure the accuracy and reliability of the detection method.

Implementation suggestions

The laboratory should regularly calibrate instruments and equipment, optimize the reagent preparation process, and strengthen personnel training to ensure the stability and consistency of the test results. It is recommended to establish standardized operating procedures and continuously improve them in combination with actual application scenarios.