GB/T 37746-2019 in English

VALID

Triplex RT-PCR assay for detection of grass carp reoviruses

Issued on:2019-06-04
Implemented on:2020-01-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $220.00
$214.00

Standard No:	GB/T 37746-2019
Document status:	VALID
Title in English:	Triplex RT-PCR assay for detection of grass carp reoviruses
Title in Chinese:	草鱼呼肠孤病毒三重RT-PCR检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-06-04
Implemented on:	2020-01-01
ICS Classification:	65.020.30-Animal husbandry and breeding
Chinese Classification:	B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification:	GB-National Standard
Related Keywords:	triple rt-pcr detection method local standards detection technology triple rt-pcr multiplex pcr high compatibility operation triplex rt-pcr assay grass carp reovirus grass carp
Related Topics:	pcr u6 in rt-pcr u6 in rt-pcr rt-pcr detection Viral Sequencing Methods pcr detection unit rt-pcr+ rt detection What does rt-pcr detect pcr method pcr method rt+pcr+ In addition to virus detection rt-pcr+2+ rt detection rt pcr pcr amplification with rt-pcr rna pcr method rt+pcr Three methods of detection of heavy metals New method for virus detection pcr virus hiv Enterovirus 68 rt square pcr pcr virus hiv 2- in rt-PCR virus detector pcr rna detection rt PCR instrument RT-PCR method to detect non- How long is rt+pcr rt pcr peak u6 in rt pcr rt pcr instrument RT-PCR detection of virus pcr detection method Enterovirus 68 rt in pcr How does pcr detect virus pcr instrument pcr method Tissue virus PCR detection what level does pcr detect rt+ pcr instrument Detection of r5 virus water quality testing pcr rt-pcr two methods pcr hiv virus Three types of heavy metal detection Prion detection method Three types of virus Virus PCR instrument Detection of pcr instrument rt-pcr relative Classification of Viruses Triorder three kinds of pcr triple virus detection method of pcr instrument enterovirus Portable RT PCR instrument Virus sequencing detection GBT37746 multiplex digital pcr virus grass carp hemorrhagic disease pcr digital PCR testing service rt-qPCR triplicate After Poisoning with Bronchus Three Laws of Viruses Virus killing behavior detection method Enterotoxins determination method Salmon testing Antiviral drug testing methods triple Enterotoxin detection method Blood parenteral toxin detection method Enterotoxin standard testing Ternary material detection method Wastewater Enterovirus Testing Methods Intestinal Adenovirus Testing

《GB/T 37746-2019草鱼呼肠孤病毒三重RT-PCR检测方法》由TC156（全国水产标准化技术委员会）归口，TC156SC11（全国水产标准化技术委员会水产养殖病害防治分会）执行，主管部门为农业农村部。

Introduction

1. Background and significance of the standard

GB/T 37746—2019 is a standard of the Technical Committee for Aquatic Products Standardization proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China, mainly for the triple RT-PCR detection method of grass carp reovirus (GCRV). The formulation of this standard fills the gap in the field of fish reovirus detection in my country and provides a scientific basis for the prevention and control of aquatic animal diseases.

2. Comparison of standard frameworks

Standard dimensions	GB/T 37746—2019	International reference standard	Comparison of local standards
Detection scope	Three genotypes of grass carp reovirus (I, II, III)	Covering a variety of aquatic animal viruses	Complementary to local standards
Detection technology	Triple RT-PCR	Multiplex PCR	High compatibility
Operation steps	One-step method and two-step method	Traditional step-by-step method	Significantly improved efficiency

3. Technical principles and reagent requirements

Technical principles:By designing specific primers for different genotypes of GCRV, three nucleic acid fragments of different sizes are amplified in the same PCR reaction system and distinguished by agarose gel electrophoresis.

Reagents and materials

M-MLV reverse transcriptase:Key enzyme for cDNA synthesis, needs to be stored at -20°C.
dNTPs:Contains 10mmol/L each of dCTP, dATP, dGTP, and dTTP.
Taq DNA polymerase:Used for PCR amplification, needs to be stored at -20°C.

4. Implementation Suggestions

To ensure the accuracy and consistency of the test, the following suggestions are made:
1. The testing agency should be equipped with PCR equipment and electrophoresis systems that meet the standard requirements.
2. The reagents must be sterilized in strict accordance with the standard requirements.
3. The operators must receive professional training and master the RT-PCR technology.

More samples: JJG 21-2008 YD/T 3934-2021 JC/T 2453-2018

Sample only — not a preview of GB/T 37746-2019

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

GB/T 39920-2021 in English

Quarantine protocol for infection with Ranavirus
2021-04-30
GB/T 20443-2006 in English

Method for the determination of diethylstilbestrol in chicken tissue by high performance liquid chromatography with electrochemical detection
2006-08-28
GB/T 36190-2018/XG1-2020 in English

Protocol of diagnostic methods for hemorrhagic disease of grass carp,includes Amendment 1
2020-12-14
GB/T 25878-2010 in English

Polymerase chain reaction(PCR) method for infectious hypodermal and haematopoietic necrosis virus(IHHNV)
2011-01-10
GB/T 28630.1-2012 in English

Diagnostic protocols for white spot disease—Part 1:Dot blot hybridization method with DNA probe
2012-07-31
GB/T 42821-2023 in English

Diagnostic methods for bonamiosis of molluscs
2023-08-06