GB/T 38580-2020 in English
VALIDTechnical specification for mutagenesis breeding of microorganisms
- Issued on:2020-03-31
- Implemented on:2020-03-31
- File Format:PDF
- Delivery:Via email within 1~3 business days
$156.00
《GB/T 38580-2020微生物诱变育种技术规范》由424-cnis（中国标准化研究院）归口，主管部门为国家市场监督管理总局。
Introduction
Interpretation of the core content of the standard
This standard systematically regulates the technical requirements for microbial mutagenesis breeding, and is applicable to the ultraviolet mutagenesis, plasma mutagenesis and chemical mutagenesis operations of microorganisms such as bacteria, actinomycetes, yeasts and molds. It focuses on making detailed provisions for the preparation of bacterial suspensions, setting of mutagenesis parameters, safety protection and other links.
Comparison of key technical indicators
| Mutagenesis type | Core parameters | Applicable microorganisms | Lethality range |
|---|---|---|---|
| Ultraviolet mutagenesis | Wavelength 260nm, Power 10-30W | Bacteria/Yeast/Mold | 75%-99.9% |
| Plasma mutagenesis | Power 100-120W, Distance 2mm | Streptomyces/Fungi | 93%-100% |
| Chemical mutagenesis | See Appendix C Concentration Gradient | Various types of microorganisms | 25%-99.7% |
Key points for implementation of the standard
Specifications for preparation of bacterial suspensions
The standard clearly requires that the concentration of bacterial suspensions be controlled between 1×106-1×108 CFU/mL, for different types of microorganisms:
- Bacteria: Collect by centrifugation during logarithmic growth phase
- Spores: Mechanically dispersed after solid culture
- Protoplasts: Require enzymatic cell wall breaking
Safety protection requirements
Testers must master the hazardous chemical handling methods specified in GB15193.19. Chemical mutagens must be used in a biosafety cabinet, and the steps after ultraviolet mutagenesis must be completed in a dark room under red light.
Technology Evolution Analysis
This standard incorporates normal pressure room temperature plasma mutagenesis technology into national standards for the first time. Compared with traditional chemical mutagenesis, it has the characteristics of no residue and high mutation rate. The lethality data provided in Appendix C show that the lethality of plasma to actinomycetes can reach 96.13%, which is significantly higher than conventional methods.
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