GB/T 39303-2020 in English

VALID

General technical specification for pre-treatment of microorganism samples in wastewater treatment system

Issued on:2020-11-19
Implemented on:2021-10-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $190.00
$185.00

Standard No:	GB/T 39303-2020
Document status:	VALID
Title in English:	General technical specification for pre-treatment of microorganism samples in wastewater treatment system
Title in Chinese:	废水处理系统微生物样品前处理通用技术规范
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-11-19
Implemented on:	2021-10-01
ICS Classification:	19.020-Test conditions and procedures in general
Chinese Classification:	G76-Basic standards and general methods for water treatment agent
Professional Classification:	GB-National Standard
Related Keywords:	wastewater treatment system introduction analysis wastewater treatment systems sewage treatment plant on-site treatment specifications microorganism samples
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Complex sample automatic pre-processing technology Arginine production wastewater treatment General Chapter 0521 Pre-processing Post-PCR sample processing National standard wastewater pretreatment GB657 Disposal of microbiologically positive samples Basic principles of sample pretreatment technology Drug sample pretreatment methods icpoes sample preprocessing icpoes liquid sample pretreatment HPLC sample preparation Atomic fluorescence water sample pretreatment Spectroscopy method sample preparation Matrix sample preparation methods Magnetic particle technical standards for water treatment Technical specifications for microcrystalline side-stream water processors Punishment for microorganisms exceeding the standard in cooked products Deep sea microbial sample preprocessing Dry ashing method pre-treatment Pretreatment methods for biological samples Selection of biological sample pretreatment methods Commonly used mass spectrometry sample preparation methods Common sample pretreatment methods and principles GC/MS pretreatment methods Pretreatment technology for chromatographic method derivatization Microplastic wastewater treatment Mining Wastewater Treatment Regulations Heavy metal sample pretreatment methods Liquid sample pretreatment methods Biofilm carrier for waste (sewage) water treatment Mass spectrometry sample preparation methods Microbial sample pretreatment system How to deal with biological waste Diazepam sample pretreatment method Fully automated sample processing system Solid sample pretreatment method Microbiological sample processing methods Solid microbial sample processing method Inorganic pretreatment methods Wastewater Sampling Technical Specifications

《GB/T 39303-2020废水处理系统微生物样品前处理通用技术规范》由TC63（全国化学标准化技术委员会）归口，TC63SC5（全国化学标准化技术委员会水处理剂分会）执行，主管部门为中国石油和化学工业联合会。

Introduction

Analysis of the core content of the standard

This standard systematically standardizes the entire process of sample pretreatment for microbial research in wastewater treatment systems, with a focus on three major technical modules:

On-site treatment specifications: clarify the collection and fixation methods of different samples such as water samples, activated sludge, and biofilm
Bacterial strain isolation technology: establish a graded separation and culture system for bacteria/actinomycetes/fungi
Metagenomic extraction: specify the quality control indicators for CTAB method DNA extraction

Comparison of key technologies

Sample type	Storage temperature	Pretreatment method	DNA extraction yield
Influent water sample	1-5℃/24h	0.22μm membrane filtration	50-200mL/membrane
Activated sludge	-20℃	Standing and stratifying	Centrifugation at 8,000rpm
Biofilm	-20℃/50% ethanol	Mechanical scraping + shaking	RNase A treatment is required

Implementation points

Typical application scenarios

A sewage treatment plant uses Membrane bioreactor process, special attention should be paid to:

The biofilm sample needs to be completely scraped from the surface of the polyvinylidene fluoride membrane with a sterile blade
The OD_260/280 ratio needs to be controlled between 1.8-2.0
Fixed samples should use 50% ethanol instead of formaldehyde to avoid DNA damage

Technology Evolution Analysis

Compared with early industry practices, this standard has the following major breakthroughs:

The gradient dilution separation method is introduced to solve the problem of separating mixed bacterial communities
The 16S/18S rRNA gene is specified as the gold standard for bacterial species identification
Establish a three-level quality control system for DNA purity (OD ratio + electrophoresis + concentration)

More samples: JJG 21-2008 JGJ 94-2008 QC/T 942-2021

Sample only — not a preview of GB/T 39303-2020