GB/T 39304-2020 in English

VALID

General technical specification for sample pre-treatment of biological toxicity detection in reclaimed water

Issued on:2020-11-19
Implemented on:2021-10-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $150.00
$146.00

Standard No:	GB/T 39304-2020
Document status:	VALID
Title in English:	General technical specification for sample pre-treatment of biological toxicity detection in reclaimed water
Title in Chinese:	再生水生物毒性检测的样品前处理通用技术规范
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-11-19
Implemented on:	2021-10-01
ICS Classification:	19.020-Test conditions and procedures in general
Chinese Classification:	G76-Basic standards and general methods for water treatment agent
Professional Classification:	GB-National Standard
Related Keywords:	standard technical links key parameters innovation points sample collection biological toxicity detection technical specification sample pre-treatment technical implementation
Related Topics:	Frontiers in sample preparation Sample pretreatment UV sample pretreatment aquatic life toxicity pre-processing sample handling Nitrogen blower sample handling biological detection biological detection Physical property detection Frontier Technology of Physical and Chemical Evidence Inspection Frontiers of Biomedical Technology biological sample moisture Biological sample din biological toxicity Sample peak forward Biological Sample Processing Methods Sample enrichment biological toxicity Biological sample processor Inorganic detection pretreatment Toxicity testing Biological Toxicity Test Physical property detection Toxicity testing saline aquatic Recycled water test sample Frontier Technology of Water Quality Testing Sample Peak Front Pre-processing test box Inorganic pretreatment detection Acute Toxicity Test Biological pretreatment equipment matrix of the sample unboiled water Biological sample reanalysis Nitrogen blower Complex sample preparation biological sample detection complex sample preparation Sample pretreatment biological sample detection method total biological sample sample pretreatment Sample front Biological Sample Processing Methods Test sample processing Sample preprocessor Sample Testing Batch Processor Application testing samples Biological Sample Processing Methods Biological toxicity standard Sample pretreatment box biological sample detection method Recultivation of Biological Samples Toxicity standard test sample biological sample detection Principles of Biotoxicity Sample preparation before testing Efficient sample preparation Detection methods of biological samples Before gas phase sample detection aquatic life The past and present of physical skills Sample pretreatment instrument Sample pretreatment Monitor sample processing Sample Handling Requirements Biological Toxicity Test pre-test processor Foreign sample pretreatment biological sample box aquatic toxic organisms prehistoric organisms Detection of Biological Samples Efficient sample preparation biological sample 17 Aquatic detection Aquatic Organism Detection toxic sample In vivo sample processing Post-test processing of samples drug testing box Test sample pretreatment g2 sample pretreatment Organic detection pre-treatment Water removal before the detector Post-test sample handling Acute Toxicity Test Toxicity detection index Acute Toxicity Toxicity Test Sample preparation and pretreatment drug testing box Pretreatment of a large number of samples Toxicity Toxicity Test Sample pretreatment testing Before sample testing To detect acute toxicity Sample Toxicology Pretreatment of samples for organic testing GBT39304 GB/T 39304-2020 Biological toxicity standard Sample pretreatment Water quality biological toxicity testing Disposal of microbiologically positive samples Basic principles of sample pretreatment technology icpoes sample preprocessing icpoes liquid sample pretreatment HPLC sample preparation Silver's biological toxicity tx100 biological sample Aquatic toxicity Bioactive substance detection technology Confocal detection of standard biological samples Treatment methods before drug detection Current methods for detecting biomarkers What are the regulatory standards for toxicity to aquatic organisms? Heavy metal detection sample pretreatment methods Selection of biological sample pretreatment methods Aquatic organism toxicity test Pretreatment technology for chromatographic method derivatization Drug testing technology Biological toxicity testing methods Cup-like virus detection technology

《GB/T 39304-2020再生水生物毒性检测的样品前处理通用技术规范》由TC63（全国化学标准化技术委员会）归口，TC63SC5（全国化学标准化技术委员会水处理剂分会）执行，主管部门为中国石油和化学工业联合会。

Introduction

Analysis of the core content of the standard

Technical links	Key parameters	Innovation points
Sample collection	Sampling point setting, container material, and labeling specifications	For the first time, the sampling requirements at the end of the recycled water network are clarified
Storage and transportation	2-8℃ refrigeration, -20℃ freezing time limit	Establish the association rules between packaging volume and storage time
Pretreatment process	Thaw mixing, component separation, pH adjustment	Introducing a dynamic adjustment mechanism for biological tolerance limits

Key points for technical implementation

The standard innovatively requires that sampling equipment use polytetrafluoroethylene-lined glass containers, and that samples containing particulate matter be subjected to standardized treatment by centrifugation at 6000 r/min for 10 minutes. pH adjustment particularly emphasizes the use of 0.1mol/L hydrochloric acid/sodium hydroxide solution to ensure that the volume change rate is less than 5%.

Typical application scenarios

Luminescent bacteria detection

It is necessary to add 3g/100mL sodium chloride to maintain osmotic pressure, and 5μm glass fiber membrane is used for filtration, which is different from the aquatic biological test method.

Solid phase extraction process

Dual column coupling technology (HLB column + coconut shell activated carbon column) achieves 1000 times concentration, flow rate control 3-5mL/min, and supporting nitrogen blower concentration requirements.