GB/T 40145-2021 in English
VALIDDetermination of 11 glucocorticoids including desonide in cosmetics—Liquid chromatography-tandem mass spectrometry
- Issued on:2021-05-21
- Implemented on:2021-12-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 40145-2021化妆品中地索奈德等十一种糖皮质激素的测定 液相色谱/串联质谱法》由TC374（全国质量监管重点产品检验方法标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
Analysis of the core content of the standard
This standard establishes a detection method for the determination of 11 glucocorticoids in cosmetics using liquid chromatography-triple quadrupole mass spectrometry. The detection limit of the method is 0.03μg/g and the quantification limit is 0.1μg/g, covering aqueous solutions, emulsions, creams (except wax-based products) and essential oil products.
Key Technical Points
| Detection Substances | Pretreatment Methods | Chromatographic Conditions | Mass Spectrometry Parameters |
|---|---|---|---|
| 11 Kinds of Desonide and Others | Essential Oils: n-Hexane Dispersion + 70% Acetonitrile Extraction Aqueous Agents: Acetonitrile Direct Extraction | C18 Column (1.7μm) 0.1% Formic Acid Water-Acetonitrile Gradient Elution | ESI Positive Ion Mode MRM Multiple Reaction Monitoring |
Method Validation Data
The method validation showed that within the concentration range of 0.1-2.5μg/g, the recovery rate was 80.8%-119.2% and the relative standard deviation was 9.9%. The qualitative ion pair of desonide was 417.1→337.1/357.2 and the collision energy was 14V.
Recommendations for the implementation of the standard
- Matrix matching calibration: blank matrix should be used to prepare the standard curve
- Mass spectrometer maintenance: regular optimization of ion source temperature (550°C) and collision gas pressure (0.06MPa)
- Quality control: blank and spiked samples should be measured simultaneously for each batch of samples
Background of technological evolution
This standard is a supplement and improvement to GB/T24800.2-2009, adding detection methods for 5 new glucocorticoids such as 16α-hydroxyprednisolone acetate, and using more advanced 1.7μm ultra-high efficiency chromatographic columns to improve separation efficiency.
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