GB/T 40447-2021 in English
VALIDDetection and identification of Rathayibacter rathayi (Smith)Zgurskaya et al.
- Issued on:2021-08-20
- Implemented on:2022-03-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 40447-2021鸭茅蜜穗病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
Analysis of the core content of the standard
The GB/T40447-2021 standard system specifies the quarantine and identification technology system of Rathayibacter rathayi. Its technical framework includes three core modules:
- Morphological identification: based on colony characteristics (light yellow, round protrusions) and microscopic morphology (Gram-positive bacilli)
- Molecular detection: Real-time fluorescence PCR using specific gsyrB gene primers
- Biological verification: Confirm the toxicity of the strain through pathogenicity testing
Comparison of key technologies
| Detection dimensions | Traditional methods | This standard method | Improved sensitivity |
|---|---|---|---|
| Pathogen isolation | Ordinary nutrient agar | D2NAX selective medium | 3-5 times |
| Identification time | 7-10 days | 72 hours (PCR) | 67% |
| Specificity | 85-90% | 99.7% (gene detection) | 14.7% |
Key points for standard implementation
Typical application scenarios
In wheat quarantine at a certain port, through seed extract centrifugation concentration combined with double PCR verification, the detection rate was increased by 22% compared with the EU standard method. Key technical parameters:
- Centrifugation speed: 14000×g
- DNA extraction amount: 10-50ng/μL
- Ct value judgment threshold: ≤35
Biosafety Specifications
Chapter 11 of the standard clearly stipulates:
- Positive samples need to be sterilized under high pressure (121℃/15min)
- Strains should be preserved by glycerol freezing method (-80℃) or lyophilization method
- Experimental waste needs to be disinfected with sodium hypochlorite (1%, 10min)
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