GB/T 40643-2021 in English

Technical background and scope of application of the standard

This standard was proposed by the State Forestry and Grassland Administration and is under the jurisdiction of the National Forest Products Standardization Technical Committee. It specifies the method for determining the content of hyperoside in hawthorn leaf extract using high performance liquid chromatography. As a flavonoid active ingredient, the content of hyperoside directly affects the quality evaluation of Chinese medicinal materials.

Core Detection Principle

Key Instrument and Reagent Specifications

Operation process quality control points

Sample processing should pay attention to: Ultrasonic extraction time is 10-30 minutes, and room temperature must be restored before constant volume. The mobile phase in the chromatographic conditions is a gradient mixture of 0.5% glacial acetic acid aqueous solution and methanol. The specific elution procedure is shown in Standard Table 1.

Method validation requirements: Linear range 0.02-1.0mg/mL, RSD＜5%, spike recovery rate 90-110%.

Implementation suggestions and common problems

1. The standard solution should be prepared before use and stored away from light
2. The filter membrane should be 0.45μm organic phase filter
3. When the sample concentration exceeds the linear range, the extraction quality should be adjusted or diluted