GB/T 40832-2021 in English
VALIDDetermination of mangiferin in mango leaves—High performance liquid chromatography
- Issued on:2021-10-11
- Implemented on:2022-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 40832-2021芒果叶中芒果苷的测定 高效液相色谱法》由424-cnis（中国标准化研究院）归口，主管部门为国家市场监督管理总局。
Introduction
Analysis of standard technical framework
| Core elements | GB/T40832-2021 specification | Conventional HPLC method |
|---|---|---|
| Detection limit | 0.75mg/g | 1.0-2.0mg/g |
| Chromatographic column type | C18 reversed column(250mm×4.6mm) | C8 or ordinary C18 column |
| Extraction efficiency | Double ultrasonic extraction (total solvent 160mL) | Single extraction |
Key technical principles
Using 258nm UV detection wavelength, based on the characteristic absorption of the benzene ring conjugated structure of mangiferin. The mobile phase uses acetonitrile-0.1% formic acid aqueous solution (15:85) system to effectively improve the peak tailing phenomenon.
Actual application case
A testing agency used this method to analyze mango leaves from Hainan production area, and measured the mangiferin content to be 12.45mg/g±0.87mg/g, RSD=2.3%, significantly better than the 15% deviation of traditional spectrophotometry.
Analysis of Standard Evolution
Compared with the 2005 version of the industry standard, this document has the following major improvements: detection limit reduced by 60%, new requirements for PTFE membrane filtration, and clear storage conditions at -18℃ away from light. This reflects the trend of improved sensitivity of HPLC detection technology and standardized sample pretreatment.
Implementation Suggestions
- When using a diode array detector, the purity of the 258nm±2nm band must be verified
- Standard solutions should be prepared and used immediately to avoid pH changes caused by formic acid volatilization
- Standards should be interspersed with each batch of samples for drift correction
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