GB/T 44614-2024 in English
VALIDInspection of grains and oils—Determination of the triacylglycerols composition in vegetable oils—High performance liquid chromatography
- Issued on:2024-09-29
- Implemented on:2025-04-01
- File Format:PDF
- Delivery:Via email within 5 business days
$301.00
《GB/T 44614-2024粮油检验 植物油中甘油三酯组成的测定 高效液相色谱法》由TC270（全国粮油标准化技术委员会）归口，TC270SC2（全国粮油标准化技术委员会油料及油脂分会）执行，主管部门为国家粮食和储备局。
Introduction
National Standard of the People's Republic of China GB/T 44614—2024
Standard Name:Grain and Oil Inspection - Determination of Triglyceride Composition in Vegetable Oils - High Performance Liquid Chromatography
Scope of Application:Applicable to the determination of triglyceride composition in vegetable oils containing long-chain fatty acid triglycerides.
Analysis of Standard Formulation Background and Technology Evolution
With the in-depth study of the nutritional components of vegetable oils, the accurate determination of triglyceride composition has become particularly important. This standard is based on high performance liquid chromatography technology, combined with advanced instruments such as differential refractive index detectors, ultraviolet detectors and evaporative light scattering detectors, and provides a variety of detection methods.
Comparison table of standard frameworks
| Chapter | Method 1 (Differential Refractive Index Detector) | Method 2 (UV Detector) | Method 3 (Evaporative Light Scattering Detector) |
|---|---|---|---|
| Reagent requirements | Chloroform, Acetone, Acetonitrile, etc. | Acetonitrile, Tetrahydrofuran | Acetonitrile, Isopropyl alcohol |
| Detector type | Differential Refractive Index Detector | >UV detector | Evaporative light scattering detector |
| Chromatographic column requirements | Stainless steel column (250mm×4.6mm) | Two C18 columns in series | Same as the first method |
Explanation of professional terms and practical application cases
Equivalent carbon number (ECN): Used to determine the peak order of triglycerides. The calculation formula ECN=CN-2n, where CN is the number of carbon atoms and n is the number of double bonds.
For example, in soybean oil, the ECN value of triolein is 50 (C18:3), while the ECN value of linolein is 46 (C18:2).
Implementation recommendations
In practical applications, it is recommended to select the appropriate method according to the type of vegetable oil:
- Differential refractive index detector is suitable for scenarios requiring high-sensitivity detection.
- UV detector is suitable for situations with special requirements for samples.
- Evaporative light scattering detector is suitable for situations requiring accurate quantitative analysis.
In addition, laboratories should regularly calibrate instruments and verify the precision and accuracy of methods to ensure that the results meet national standard requirements.
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