GB/T 45136-2024 in English
VALIDTechnical specification for somatic cell cloning of pig, cattle, sheep and goat
- Issued on:2024-12-31
- Implemented on:2024-12-31
- File Format:PDF
- Delivery:Via email within 5 business days
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《GB/T 45136-2024猪牛羊体细胞克隆技术规范》由424-cnis（中国标准化研究院）归口，主管部门为市场监管总局。
Introduction
In-depth interpretation of the technical specifications for somatic cell cloning of pigs, cattle and sheep
1. Background and significance of the standard
GB/T 45136-2024 "Technical Specifications for Somatic Cell Cloning of Pigs, Cattle and Sheep" is an important national standard that aims to regulate the application of somatic cell nuclear transfer technology in economic animals such as pigs, cattle and sheep. The standard was jointly drafted by a number of authoritative institutions including the Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, and fully considered the latest progress in related research at home and abroad.
2. Comparison of standard frameworks
| Dimensions | Pig somatic cell cloning | Bovine somatic cell cloning | Sheep somatic cell cloning |
|---|---|---|---|
| Oocyte collection | A.1 | B.2 | C.2 |
| In vitro maturation culture | 44-46h | 18-22h | 18-22h |
| Nuclear transfer operation | Electrofusion method | Microelectrode fusion | Electrofusion |
3. Core steps of nuclear transplantation technology
Case study: Operational procedures of porcine somatic cell cloning
- Oocyte collection and selection: Select COCs with a diameter of $3\sim6\,\mathrm{mm}$.
- In vitro maturation culture: Culture for $44\sim46\,\mathrm{h}$ at a CO2 concentration of $5\%$.
- Enucleation and nuclear injection: Use a micromanipulation system to remove polar bodies and inject donor cells.
- Electrofusion activation: Somatic cells and oocytes are fused by electrical stimulation.
4. Implementation Suggestions
To ensure the successful implementation of somatic cell cloning technology, it is recommended that:
- Strictly follow the standard operating procedures for experiments.
- Select high-quality reagents and culture media (as shown in Tables A.1, B.1, and C.1).
- Strengthen technical training for laboratory personnel, especially micromanipulation skills.
- Establish a complete quality control system to ensure the traceability of each batch of experiments.
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