GB/T 45271-2025 in English
VALIDMan-made fiber—Determination of catechins content—High performance liquid chromatography
- Issued on:2025-02-28
- Implemented on:2025-09-01
- File Format:PDF
- Delivery:Within 1 day
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《GB/T 45271-2025化学纤维 儿茶素含量的测定 高效液相色谱法》由TC586（全国化学纤维标准化技术委员会）归口，主管部门为国家标准委。
Introduction
Technical background and significance of the standard
With the growing demand for functional textiles, catechin-modified fibers are widely used due to their antibacterial and antioxidant properties. This standard establishes for the first time a method system for the determination of five catechin monomers in fibers by high performance liquid chromatography, filling the gap in industry testing.
Core method principle
Methanol extraction-ultrasonic assisted extraction technology is used, separated by Polar Advantage II chromatographic column (4.6mm×250mm×5μm), and quantified by external standard method. The key steps include:
| Technical links | Parameter requirements | Quality control points |
|---|---|---|
| Sample pretreatment | 4.8-5.2g sample, 0.2-0.5cm fragments | GB/T 6504 degreasing treatment |
| Ultrasonic extraction | 150mL methanol, 30min/400W | 12h pre-soaking |
| Chromatographic conditions | Flow rate 1.0mL/min, column temperature 30℃ | Gradient elution program |
Key instrument configuration requirements
High performance liquid chromatograph needs to be equipped with a diode array detector. The supporting equipment includes:
- Ultrasonic cleaner: ≥400W power, 40kHz frequency
- Rotary evaporator: 35±5℃ water bath temperature control
- Microporous filter membrane: 0.45μm organic phase filter membrane
Method performance indicators
The standard stipulates strict detection limit and precision requirements:
| Compound | Detection limit (μg/kg) | Quantitative limit (μg/kg) |
|---|---|---|
| (+C) | 100 | 400 |
| EGCG | 80 | 280 |
| ECG | 60 | 200 |
The repeatability limit is set at 200μg/kg, and the probability of exceeding is ≤5%
Implementation suggestions and precautions
- Selection of standard substances: It is recommended to use CRMs standards with a purity of ≥98%
- Mobile phase preparation: Ammonium formate buffer system needs to be accurately weighed 0.0631g
- Temperature control: The rotary evaporation process is strictly maintained at 35±5℃
- Data verification: Standard curve correlation coefficient R²＞0.99
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