MZ/T 140-2019 in English
VALIDTechnical specifications for examination ofpathogenic bacteria in funeral home
- Issued on:2019-12-12
- Implemented on:2019-12-12
- File Format:PDF
- Delivery:Via email within 1~3 business days
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本标准规定了各类殡仪场所致病菌的采样及检测检验方法。
本标准适用于殡仪馆、火葬场、骨灰堂、公墓、殡仪服务站、殡仪车等固定和流动殡仪场所。
Introduction
Standard Background and Scope of Application
This standard was proposed by the Ministry of Civil Affairs and is under the jurisdiction of the National Technical Committee for Funeral Standardization. It is a technical specification for microbial testing formulated for the special environment of funeral venues. It is applicable to the monitoring of pathogenic bacteria in fixed and mobile funeral service venues such as funeral homes, crematoria, and columbariums.
Comparison of core detection indicators and methods
| Test items | Sampling method | Culture medium | Culture conditions | Judgment criteria |
|---|---|---|---|---|
| Total bacteria count | Impact method/natural sedimentation method | Nutrient agar | 36±1℃ 48h | Maximum value of cfu/m³ |
| Hemolytic Streptococcus | Impact method | Blood agar plate | 36±1℃ 24-48h | β-hemolytic ring + Gram staining |
| Escherichia coli | Cotton swab method | Lactose bile salt medium | 36±1℃ 24h | Acid and gas production + microscopy |
Analysis of key technical points
1. Total bacterial count detection
Use a six-stage sieve impact sampler (flow rate 28.3L/min) or natural sedimentation method:
- Sampling point layout: 3 points for ≤30m², 5 points for ＞30m², 1m away from the wall
- Calculation formula: Q=N/(L×T)×1000 (Q:cfu/m³)
2. Identification of hemolytic streptococci
Typical case: Colony characteristics found in the rest area of a funeral home:
- Gray-white translucent colonies (0.5-0.7mm)
- Gram-positive chain arrangement
- 2-4mm complete hemolysis ring
Recommendations for the implementation of the standard
- Equipment such as the autoclave must be calibrated before sampling
- The pH value of the culture medium must be strictly controlled in the range of 7.2-7.6
- Fresh defibrinated sheep blood must be used for the hemolysis test
- It is recommended to conduct a comprehensive test once a quarter
Technology evolution analysis
Compared with GB19053-2003, this standard has the following major improvements:
- New requirements for testing mobile places such as funerals
- Refine the height specification of sampling points (1.2-1.5m)
- Introduce Baird-Parker medium for identification of Staphylococcus aureus
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