NY/T 564-2016 in English
VALIDDiagnostic techniques for swine pasteurellosis
- Issued on:2016-10-26
- Implemented on:2017-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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| Standard No: | NY/T 564-2016 |
| Document status: | VALID |
| Title in English: | Diagnostic techniques for swine pasteurellosis |
| Title in Chinese: | 猪巴氏杆菌病诊断技术 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2016-10-26 |
| Implemented on: | 2017-04-01 |
| Superseding: |
NY/T 564-2002 Diagnostic techniques for swine pasteurellosis
|
| Professional Classification: | NY-Agriculture |
| Related Topics: | diagnostic technology
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Introduction
Background of Standard Revision and Technological Evolution
The 2016 version of the standard has undergone major technical upgrades based on the 2002 version, mainly reflected in:
| Technical Dimensions | 2002 Version | 2016 Version |
|---|---|---|
| Pathogen Identification Methods | Traditional Biochemical Identification | Newly Added PCR Typing Technology |
| Capsule Typing Technology | Indirect Hemagglutination Test | Added Multiplex PCR Typing Method |
| Culture Medium System | Improved Martin Agar | Switch to tryptic soy agar medium |
Key points of core diagnostic technology
1. Clinical diagnostic features
- Most acute type: sudden death, swollen neck, dog-like sitting posture
- Acute type: high fever (41-42℃), dyspnea, marbled lung lesions
- Chronic type: persistent cough, swollen joints, progressive weight loss
2. Pathogen isolation and identification
Use Tryptic soy agar medium (TSA) for isolation and culture. Key identification indicators are:
| Test items | Characteristic manifestations |
|---|---|
| Microscopic characteristics | Gram-negative coccobacillus, densely stained at both poles |
| Biochemical characteristics | Glucose+, sucrose+, urease- |
| PCR identification | 460bp kmt1 gene fragment |
Serotype identification technology upgrade
Comparison of dual detection systems
| Technical parameters | Indirect hemagglutination test | Multiplex PCR method |
|---|---|---|
| Detection cycle | 24-48h | 4-6h |
| Typing ability | Type A/B/D | Type A/B/D |
| Specificity | 90-95% | >99% |
Note: Multiplex PCR amplification fragment: Type A 1044bp, Type B 760bp, Type D 657bp
Recommendations for the implementation of the standard
- Laboratories should establish operating specifications for Class II biosafety cabinets
- TSA/TSB medium is recommended to replace traditional Martin medium
- 18-22g healthy mice should be used for virulence determination
- A positive and negative control system should be set up for PCR detection
Appendix Application Guide
The standard appendix contains 13 media preparation methods, pay attention to:
- Tryptic Soy Agar (TSA): 0.1% sheep lysed blood cells should be added
- Electrophoresis buffer (TAE): 50× storage solution should be kept away from light
- Sugar fermentation tube: an inverted small tube should be built in to observe gas production
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