SC/T 7228-2019 in English
VALIDCode of diagnosis for infectious myonecrosis (IMN)
- Issued on:2019-08-01
- Implemented on:2019-11-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$185.00
Introduction
Analysis of the core content of the standard
| Detection dimensions | Histopathology | Nested RT-PCR | Comprehensive judgment |
|---|---|---|---|
| Sensitivity | Depends on the extent of the lesion | Can detect 1-1000ng RNA | Double-positive diagnosis |
| Specificity | Needs to be differentiated from PvNV | 139bp/328bp double verification | Sequence alignment confirmation |
| Timeliness | 48 hours (including sections) | 6-8 hours | Grading |
Key technical points
1. Histopathological diagnosis
Typical features: Coagulative necrosis of striated muscle with hemocyte infiltration, hypertrophy of lymphoid organs (3-4 times). It is necessary to distinguish it from white tail disease caused by Lopenaeus vannamei Nodamura virus (PvNV).
2. Nested RT-PCR operation
- Primer design:Use two pairs of specific primers (4587F/4914R→4725NF/4863NR)
- Amplification product:328bp in the first step→139bp in the second step
- Anti-pollution measures:DEPC water treatment consumables are required, and a blank control should be set
Recommendations for standard implementation
Application scenario example
The tail fan of Penaeus vannamei shrimp in a farm showed symptoms of bleaching:
1. First, perform histopathological sections to observe the characteristics of muscle fiber necrosis
2. Simultaneously collect muscle samples for nested RT-PCR detection
3. Isolation measures are initiated after double positive results confirm IMN
Technology evolution analysis
Compared with the earlier version, this standard has been upgraded mainly as follows:
- Added two-step RT-PCR to improve detection sensitivity
- Clarified the pathological characteristics of lymphoid organ spheroids (LOS)
- Standardized sampling sites (whole sample for larvae and muscle/gill/hemolymph for adult shrimp)
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