SC/T 7232-2020 in English
VALIDCode of diagnosis for Enterocytozoon hepatopenaei disease
- Issued on:2020-08-26
- Implemented on:2021-01-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$185.00
| Standard No: | SC/T 7232-2020 |
| Document status: | VALID |
| Title in English: | Code of diagnosis for Enterocytozoon hepatopenaei disease |
| Title in Chinese: | 虾肝肠胞虫病诊断规程 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2020-08-26 |
| Implemented on: | 2021-01-01 |
| Professional Classification: | SC-Aquaculture |
| Related Topics: | liver
shrimp cell Gut parietal cells intestinal diagnostic regulations Bug disease diagnostic reagents and shrimp liver disease diagnosis Pathological remote diagnosis Liver+ sick shrimp Foreign Pathological Diagnosis Diagnosis Routine Diagnosis intestinal% Diagnosis of Pupa Hepatitis D and Hepatitis E Diagnosis Standard SC/T Enterohepatic fgf12 Liddell's liver disease Enterobacteriaceae detection Diagnosis of intestinal hernia Kikuchi disease diagnosis Diagnosis of intussusception |
Introduction
Analysis of the core content of the standard
| Detection method | Sensitivity | Specificity | Time consumption | Applicable scenarios |
|---|---|---|---|---|
| Histopathology | Medium | High | 4-6 hours | Laboratory confirmation |
| Nested PCR | 102 copies/μL | Extremely high (SWP gene) | 3 hours | Early Screening |
| TaqMan Fluorescence Quantitative PCR | 101 copies/μL | Medium (SSU rRNA gene) | 2 hours | Batch Testing |
Key Technical Points
1. Sample Processing Specifications
The standard requires differentiated sampling for different growth stages of hosts such as L. vannamei:
- Larval stage: take complete individuals
- Adult stage: take hepatopancreas/intestinal tissues
- Bait organisms: combined sample testing is required
2. Molecular Detection Innovation
Nested PCR design features:
Using SWP gene-specific primers (514F/R→147F/R), the detection limit is reduced to 100 copies/μL through two-step amplification to avoid cross-reaction.
Fluorescence quantitative PCR precautions:
When using SSU rRNA universal primers, the results of nested PCR should be combined to exclude interference from other enterocytotic parasites, and the standard curve efficiency should be 88.9%-110% (R²≥0.995).
Implementation recommendations
1. Optimization of the detection process
- Initial screening stage: TaqMan fluorescent quantitative PCR (high throughput) is preferred
- Confirmation stage: Histopathological verification is required
- Seedling detection: It is recommended to combine nested PCR and fluorescent quantitative PCR
2. Laboratory quality control
The standard emphasizes the three-control principle:
- Positive control (known EHP-DNA)
- Negative control (healthy shrimp tissue)
- Blank control (sterile water)
Analysis of standard evolution
Compared with previous methods, the main breakthroughs of SC/T 7232-2020 are:
1. Introducing TaqMan probe technology to achieve quantitative detection
2. Clarify the standardized process of DNA extraction by CTAB method (recovery rate 60%)
3. Establish a correlation determination system between clinical symptoms and laboratory tests
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