SC/T 9433-2019 in English

VALID

General rules for description of fishery germplasm resource

Issued on:2019-08-01
Implemented on:2019-11-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $90.00
$88.00

Standard No:	SC/T 9433-2019
Document status:	VALID
Title in English:	General rules for description of fishery germplasm resource
Title in Chinese:	水产种质资源描述通用要求
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-08-01
Implemented on:	2019-11-01
Professional Classification:	SC-Aquaculture
Related Keywords:	fishery germplasm resource introduction analysis germplasm resources implementation germplasm resources crab germplasm genetic analysis chromosome analysis
Related Topics:	Germplasm resources Aquatic products Germplasm resources efficacy description Germplasm Partial water quality of several kinds of water Water quality description Hair Requirements Describe water quality Water resources demonstration does not require qualifications PCR water requirements quality Silica resource definition

Introduction

Analysis of the core content of the standard

Module	Old standard from the 1990s	SC/T 9433-2019 new regulations	Technical upgrade points
Genetic analysis	Chromosome analysis only	Added 7 new molecular markers such as SSR/SNP/DNA barcodes	Application of genome sequencing technology
Sample information	Simple morphological record	Standardized coding system + 3D image acquisition	Digital management requirements
Nutritional components	Basic protein and fat detection	Fatty acid/amino acid full spectrum analysis	Introduction of mass spectrometry technology

Detailed explanation of key technical requirements

4.1 Code system specifications

A composite coding structure of species category (2 digits) + species name pinyin initials + preservation form code + timestamp is adopted, for example: 01-CP-03-20191101 represents live preserved samples of Cyprinidae fish.

7.7 Microsatellite DNA analysis

Must be tested in accordance with the provisions of GB/T 34748:

Number of alleles at each locus ≥ 4
Polymorphic information content PIC＞0.5
Length of amplified fragment 100-300bp

Implementation and application suggestions

Case: Identification of Chinese mitten crab germplasm

Using the DNA barcoding technology in Clause 7.9 of this standard, through COI gene sequence comparison (primers: LCO1490/HCO2198), the genetic distance between the Yangtze River population and the Liaohe River population was successfully identified as 0.032, providing a molecular basis for the protection of germplasm resources.

Background of Standard Evolution

With the development of aquatic breeding technology, the original standard can no longer meet the following requirements:

Application requirements of new technologies such as whole genome selection
Standardization requirements for cross-border exchange of germplasm resources
Implementation of the International Convention on Biological Diversity Conservation

More samples: QC/T 942-2021 DL/T 5154-2012 YD/T 3934-2021

Sample only — not a preview of SC/T 9433-2019