SN/T 1538.1-2016 in English

VALID

Guidelines on preparation and production of culture media-Part 1:General guidelines on quality assurance for the preparation of culture media in the laboratory

Issued on:2016-08-23
Implemented on:2017-03-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $380.00
$369.00

Standard No:	SN/T 1538.1-2016
Document status:	VALID
Title in English:	Guidelines on preparation and production of culture media-Part 1:General guidelines on quality assurance for the preparation of culture media in the laboratory
Title in Chinese:	培养基制备指南第1部分：实验室培养基制备质量保证通则
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2016-08-23
Implemented on:	2017-03-01
Superseding:	SN/T 1538.1-2005 Guidelines on preparation and production of culture media - Part 1 : General guidelines on quality assurance for the preparation of culture media in the laboratory
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	culture media transport/preservation/suspension culture media solid culture media liquid culture media semi-solid culture media
Related Topics:	Microplate nourish mass culture culture medium Media UV Double antibody in culture medium Media system Media preparation instrument Medium double antibody Media UV pH of medium Medium pH senior culture medium tcbs medium Preparation of ts medium Medium+ culture medium + ph double antibody in culture medium double antibody Culture medium equipment How to make culture medium Medium selection ts medium Media products culture tray Media filter Medium secondary antibody Preparation amount efficient medium Double antibody in culture medium growth medium Medium double antibody Media Preparer e8 medium Medium pH e6 and e8 media UV medium Double Antibody Medium Medium content Medium volume anti-medium laboratory semi-preparation ph medium Medium sample divider medium orange Animal medium double antibody Medium volume Culture medium instrument co-culture experiments Automatic culture medium preparation instrument gelatin medium Media Preparer Medium double antibody 2% m2 medium cod culture culture medium Double antibody in culture medium Medium storage time laboratory preparation Three Antibody Medium m4 medium Medium storage time laboratory preparation m7 medium Culture medium three antibodies conditioned medium time medium medium+ph Content of t2 medium All-in-one Media Preparer Double antibody in culture medium Automatic medium preparation instrument chemical medium Culture medium instrument brain heart infusion medium Culture medium preparation Which culture medium is broad-spectrum Culture medium detection Culture medium preparation machine broad-spectrum medium w5 medium laboratory culture flask Chapi Medium Spore culture medium Automatic Media Preparer culture medium+ph+ Culture medium preparation Media Automatic Preparer h9 medium Preparation Guide Preparation ts medium Five-level culture medium pre-prepared media tank medium Test equipment maintenance collagen medium n6 medium medium c2 anti + medium c2 medium m1 medium Medium time Medium ts Material medium intermediate medium Medium ts f2 medium preservation medium palm m9 medium plate agar medium Medium + filter How to prepare a culture medium culture medium prepared quality medium dish culture Shelf life verification culture medium against medium + reverse My luxuriant medium surface culture basic medium Cultivation Culture medium experiment Culture medium preparation double medium Analytical medium conditioned medium medium indicator Indicators in culture medium Culture medium + indicator Medium substance detection semi-solid medium Conditioned medium experiment Medium M9 Double antibody in culture medium culture medium V8 liquid medium medium e8 Medium storage medium indicator dehydrated medium medium surface gelatin medium Medium density gelatin + culture medium Culture medium three antibodies Medium diameter culture medium How to control the culture medium Microbiological Media Preparation Guidelines Indicators in culture media Culture medium production Media for Specific Indicators Medium canned Medium density Culture medium and double antibody Culture medium and preparation Control Medium Storage "Cultivation Medium Media Preparer How to control the quality of medium Media Preparation Analysis Media transplant Media Growth Inhibition Gelatin Medium+ Medium quality surface medium microbiological laboratory culture medium Medium quality air medium Reagents for preparing media Culture medium experiment laboratory incubator Culture medium commonly used Microbial Preparation Medium Media Control Bacterial culture medium Culture medium three grade water laboratory medium Media Test Medium diameter ammonium medium About Automated Media Preparers General quality control of media Medium glutamax hep3b medium lb medium formula Preparation method of TB culture medium tb medium formula tpy medium shelf life LB medium and 2YT Maggots growing in culture medium Infectious culture guide Medium turns yellow Classification of culture media and characteristics of various culture media bp medium f12k medium turns yellow yps medium and YPD The concentration of callus medium ms medium Actipro culture medium preparation method pdb medium How to prepare plate culture medium Preparation of BL medium before experiment EMEM medium Liquid culture medium quality Liquid culture medium quality Media industry Media acceptance Blood culture manufacturing, testing and acceptance standard medium Media acceptance quality Training is golden Standard curing room control equipment Preparation method of milk culture medium Media configuration Preparation method of Cha's culture medium How to prepare conditioned medium Are there quality standards for culture media? preparation Media industry Preparation method of ampicillin culture medium Conditioned medium method co-culture

Introduction

Analysis of the core content of the standard

Technical elements	2005 edition	Main changes in the 2016 edition
Scope of application	Basic laboratory requirements	Expanded to three types of culture media: commercial ready-to-use, dehydrated synthetic and component prepared
Definition of terms	23 items	9 new definitions such as transport/preservation/suspension culture media, totaling 32 items
Quality control strain management	Secondary management system	Establish a three-level system of standard strain → reserve strain → working strain

Detailed explanation of key technical requirements

1. Culture medium classification system

The standard divides culture media into liquid culture media, semi-solid culture media and solid culture media according to their physical state. The agar content of solid culture media must be controlled at 1.2-1.5%.

Selective culture medium: such as XLD agar, the bile salt inhibition amplitude should be more than 85%
Identification culture medium: the lactose fermentation reaction of MacConkey agar needs to develop color within 18-24h
Complex functional culture medium: such as blood agar, which has both recovery and identification characteristics

2. Key points of quality control in the preparation process

Key parameter control table

Link	Control index	Allow deviation
Rehydration	Conductivity	≤25 μS/cm（25℃）
Sterilization	Temperature/time	121±1℃/15min
pH adjustment	Difference before and after sterilization	±0.2 unit

Typical case: After boiling, the intestinal bacteria culture medium needs to be quickly cooled to below 50℃ to avoid bile salt decomposition

Recommendations for the implementation of the standard

Establish a culture medium batch number traceability system, and record the receipt date, opening time and sensory inspection results
Implement repackaging and freezing management for heat-sensitive additives (such as antibiotics), and the shelf life after thawing shall not exceed 7 days
Adopt the three-level strain preservation method: standard strain (lyophilized) → reserve strain (-70℃) → working strain (4℃)

Technology evolution analysis

The main differences between this standard and ISO TS 11133-1:2009 are:

Added the special requirements for water quality testing of Chinese Pharmacopoeia
Strengthened the acceptance clauses for commercial ready-to-use culture media
The troubleshooting provided in Appendix B is unique content

More samples: NB/T 47013.2-2015 QC/T 942-2021 GB 50021-2001(2009)

Sample only — not a preview of SN/T 1538.1-2016

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