SN/T 3177-2012 in English
VALIDDetection and identification of Ophiostoma wageneri(Goheen & Cobb)Harrington
- Issued on:2012-05-07
- Implemented on:2012-11-16
- File Format:PDF
- Delivery:Via email within 1~3 business days
$272.00
Introduction
Technical Background of the Standard
This standard is a quarantine identification method for Opjiostoma zaagereri, which originated from the devastating damage of the pathogen to pine and fir plants in North America. In 2012, the Chinese quality inspection system established a special inspection standard for the first time, filling the technical gap in the quarantine of conifer root diseases in China.
Comparison of core detection elements
| Detection dimensions | Pine black root pathogen | Blue stain bacteria |
|---|---|---|
| Lies spot morphology | Crescent-shaped concentric rings | Radial fan-shaped |
| Hypha distribution | Longitudinal growth of tracheids | Thin-walled cell aggregation |
| Culture characteristics | Slow growth on PDA (turns black in 7 days) | Rapidly expanding colonies |
Key points for the implementation of key technologies
Field sampling specifications
It is necessary to collect 20-30cm wood segments together with the vector insects (such as black fat beetles), and focus on observing three types of section characteristics:
- Cross-section: Look for black crescent-shaped lesions
- Radial section: Check for longitudinal black stripe lesions
- Chordal section: Confirm conidiophores in the insect tunnels
Laboratory identification process
Use the double verification method:
- Microscopic examination: Observe the characteristics of hyphae penetrating the marginal pits
- Cultivation: PDA selective medium (containing 0.2g/L actinomycin) at 18℃ for 3 days
Standard application case
In 2018, Tianjin Port detected suspected cases in imported Douglas fir logs through:
- Comparison of conidiophore morphology (30-940μm×10-12μm)
- Verification of the actinomycin resistance of mycelium
- PCR gene sequencing confirmation
It was finally determined to be the Ophiostoma wageneri var. pseudotsugae variant, successfully blocking the introduction of the epidemic.
Technology Evolution Analysis
Compared with the 2005 EPPO standard, this standard adds:
- Isolation and culture methods for bacteria carried by insect vectors
- Precise formula of PDA selective culture medium (Appendix C)
- Morphological differentiation indicators for the three variants
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