SN/T 4715-2016 in English
VALIDRegulation for collection，making and preservation of weed voucher specimens
- Issued on:2016-12-12
- Implemented on:2017-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$156.00
| Standard No: | SN/T 4715-2016 |
| Document status: | VALID |
| Title in English: | Regulation for collection，making and preservation of weed voucher specimens |
| Title in Chinese: | 杂草凭证标本采集、制作与保存规范 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2016-12-12 |
| Implemented on: | 2017-07-01 |
| Professional Classification: | SN-Import&Export Inspection |
| Related Keywords: | weed specimens
preservation management requirements preservation environment control herbara rapid preservation kit weed voucher |
| Related Topics: | Impurity verification
Calibration set and validation set specimen preparation method specimen preservation box specimen preservation box How to make a specimen Collecting specimens in the field The role of the validation set Validation set role Oxalic acid powder storage Specimen collection industry Collection and storage of sludge |
Introduction
Standard Overview and Technical Background
SN/T 4715-2016 is my country's first industry standard for the full lifecycle management of weed specimens in the entry-exit inspection and quarantine sector. It is based on GB/T 1.1-2009, the Guidelines for Standardization. This standard, for the first time, combines DNA barcoding technology with traditional specimen preparation requirements, reflecting the development trends of modern plant taxonomy.
Analysis of core technical requirements
2.1 Specimen collection specifications
| Collection elements | Traditional requirements | DNA supplementation requirements |
|---|---|---|
| Collection quantity | Complete plants or branches over 30cm | Add 3-5g fresh tissue |
| Record information | Geographic coordinates, habitat characteristics | Add DNA extraction status record |
2.2 Specimen processing technology
Special provisions of the standard DNA materials require a two-step treatment: pre-fixation with 70% ethanol followed by rapid drying on silica gel to ensure DNA fragment integrity. Comparative experiments have shown that this method can increase DNA extraction success rates by over 40%.
Preservation management requirements
4.2 Preservation environment control
- Herbara specimens: temperature 15-25℃, humidity 40-50%
- DNA materials: long-term storage at -20℃ or ultra-low temperature storage in liquid nitrogen
- Specimen cabinets must be equipped with insect-proof camphor preparations
Implementation suggestions
- When collecting in the field, be equipped with a portable dryer and a DNA rapid preservation kit
- Establish a specimen-gene dual numbering system
- Regularly monitor the temperature and humidity fluctuations of the storage environment
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