SN/T 4727-2016 in English

VALID

Detection and identification of Nacobbus aberrans

Issued on:2016-12-12
Implemented on:2017-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	SN/T 4727-2016
Document status:	VALID
Title in English:	Detection and identification of Nacobbus aberrans
Title in Chinese:	异常珍珠线虫检疫鉴定方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2016-12-12
Implemented on:	2017-07-01
Professional Classification:	SN-Import&Export Inspection
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Introduction

Technical Background of the Standard

SN/T 4727-2016 is my country's first special testing standard for pseudo-root-knot nematode (Nacobbus aberrans), filling the gap in quarantine nematode identification technology.

Key points of morphological identification

Characteristic parameters	Pseudo-root-knot nematodes	Similar species	Microscopic observation magnification
Body length of female (μm)	710-930	300-380	400×
Style length (μm)	21-25	18-26	1000×
Vulva position (%)	92-94	85-90	400×

Molecular detection technology

The standard adopts the PCR primer system of SN/T 2757:

Forward primer: 5'-CGTGGAATGATCAGAACGC-3'
Reverse primer: 5'-TCCCAAAGACCTTCATTCTTC-3'

The expected length of the amplified product is 240bp, and the annealing temperature is 58℃.

Host Plant Range

Ruboid knot nematodes can infect over 100 plant species across 24 families, primarily including:

Solanaceae: potato, tomato, pepper
Cucurbitaceae: cucumber, pumpkin
Cruciferae: cabbage, rapeseed
High-risk quarantine hosts: beet, carrot

Recommendations for Implementation of the Standard

1. Sample Storage: Refrigerate at 4°C for no more than 3 days; long-term storage requires freezing at -20°C.
2. Morphological identification requires measurement of at least 20 morphological parameters.
3. Molecular testing should include a positive control (ATCC 49236).
4. Quarantine treatment: soil fumigation is required if an epidemic is found (methyl bromide 40g/m³ is recommended)

More samples: JC/T 2453-2018 JGJ 94-2008 DL/T 5154-2012

Sample only — not a preview of SN/T 4727-2016

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