SN/T 5103-2019 in English

VALID

Detection method of bio-toxicity by Luminescent bacteria test for drinking water at frontier port

Issued on:2019-09-03
Implemented on:2020-03-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $100.00
$97.00

Standard No:	SN/T 5103-2019
Document status:	VALID
Title in English:	Detection method of bio-toxicity by Luminescent bacteria test for drinking water at frontier port
Title in Chinese:	国境口岸饮用水生物毒性发光细菌检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-09-03
Implemented on:	2020-03-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	detection method luminescent bacteria test rapid detection method luminescent bacteria parallel sample detection data
Related Topics:	How long does drinking water test Drinking Water Testing Method Bacteria detection method Bacteria detection method in water Bacteria detection Water detection method Biological Toxicity Test Method Drinking Water Testing Method Testing methods for drinking water Bacteria detection Water Quality Bacteria Detection Method Methods for the detection of microorganisms in drinking water Methods for the detection of microorganisms in drinking water Microorganism detection method in drinking water Microorganism detection method for drinking water 20 Bacteria detection box Bacteria detection by flow cytometry Water Quality Bacteria Detection Method Detect 19 items of drinking water Microorganism detection method in drinking water Drinking water testing method Non-drinking water testing Bacterial microorganism detection method Bacterial testing method for water quality Drinking Water Self-Test Aquatic Toxicity Test Method Biological Toxicity Test Method Bacterial Molecular Biology Detection 42 tests for drinking water Sulfur bacteria detection method Physical method Bacteria Bacteria detection substance Bacterial testing method for water quality national bacteria Photoelectric detection abroad 19 tests for drinking water Bacterial growth assay method Water Bacteria Detection Method Bacterial microorganism detection method Methodology Bacterial Detection Test methods for detecting bacteria Detection methods for indicators in drinking water Water quality biological toxicity testing Water quality toxicity testing method Luminous Bacteria International Chinese Pharmacopoeia bacterial endotoxin detection method Chinese Pharmacopoeia Bacterial Endotoxins Test Method U.S. drug testing methods Soil biological toxicity detection method Bacterial homology analysis and detection methods Standard testing methods for bacteria Detection method of photogenerated holes Luciferase method for detecting bacteria Colorimetric method for testing drinking water National standard detection method for mercury in water The easiest way to detect bacteria What are the regulatory standards for toxicity to aquatic organisms? French fungal detection method Applicability of sterility testing methods Commonly used methods for bacterial detection What is the national standard for bacterial detection? Bacterial biofilm staining methods Traditional bacterial detection methods Purified water bacterial detection method New method for detecting life effects Bacteria national standard detection method Biomass power generation abroad Aluminum detection method for drinking water General testing method for bacteriological testing Testing methods for sterile water National drinking water quality testing How to monitor bacteria in biological safety cabinets Testing methods for wastewater toxicity IVD luminescence method Biomarker detection method development drinking water toxicity testing group Comparison of microbial detection methods in pharmacopoeias of various countries Mecon Bioluminescence Methodology Hand bacteria detection method International biochemical identification of bacteria Main methods for determining bacterial virulence Biological toxicity testing methods Ring Flexibility Test Method International

Introduction

Technical background of the standard

This standard is based on the biosensing characteristics of luminescent bacteria to establish a rapid detection method for the comprehensive toxicity of chemical pollutants in drinking water at ports. Compared with traditional chemical analysis, this method has technical advantages such as 15-minute rapid response and comprehensive toxicity assessment.

Core Detection Principle

Technical Elements	Parameter Requirements	Biological Basis
Indicator Bacteria	3 species including Vibrio fischeri (V.fischeri)	Luciferase system in bacteria is sensitive to toxic substances
Detection Wavelength	490nm	Optimum Bioluminescence Spectrum
Key Indicators	Relative Luminescence Index (RLI)	Luminescence Inhibition Rate Reflects Toxicity Intensity

Instrument and Reagent Requirements

Typical equipment configuration:It needs to be equipped with a LumiFox2000 bioluminescence detector, and its temperature control accuracy must reach ±0.5℃. The reagent system includes 7 types of special solutions such as 3% sodium chloride activator and 30% stop solution.

Quality control:The blank control RLU value should be ≥800, otherwise the bacterial activity or instrument status needs to be checked.

Key points of the operation process

Sample pretreatment: Store at 2-8℃ for no more than 24 hours, avoid light
Reaction system: 900μL sample + 100μL bacterial suspension, oscillate and mix for 15s
Detection sequence: Read the initial RLU value at 4min, calculate the RLI at 5min

Result judgment standard

When the RLI value of the sample decreases by ≥15% compared with the blank control, it is judged as a positive result, which needs to be combined with the parallel sample detection data (coefficient of variation <10%). GC-MS confirmation analysis should be performed in special cases.

Implementation suggestions

Establish a regular culture subculture system to ensure the activity of ATCC standard strains
Each batch of tests must include a positive control (3,5-dichlorophenol)
Form a complementary testing scheme with the conventional indicators of GB5749

More samples: GB 18306-2015 YD/T 3934-2021 DL/T 368-2010

Sample only — not a preview of SN/T 5103-2019

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