SN/T 5120-2019 in English

VALID

Determination of nitrite in edible animal and feeds for import and export - Colorimetric analysis and ion chromatography

Issued on:2019-09-03
Implemented on:2020-03-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $90.00
$88.00

Standard No:	SN/T 5120-2019
Document status:	VALID
Title in English:	Determination of nitrite in edible animal and feeds for import and export - Colorimetric analysis and ion chromatography
Title in Chinese:	进出口食用动物、饲料中亚硝酸盐测定比色法和离子色谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-09-03
Implemented on:	2020-03-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	ion chromatography introduction analysis ion chromatography requires as19 mg/kg spectrophotometer ion chromatography complex matrix samples mg/kg ion chromatograph key points gradient elution chromatography technology
Related Topics:	Determination of Sulfite in Water by Ion Chromatography Role in Ion Chromatography How to measure ion chromatography Ion Chromatography Nitrate Peak Determination of nitrate in water quality, ion chromatography Colorimetric method for determination of sulfide ion Determination of sulfites and bisulfites in cosmetics for import and export by ion chromatography Determination method of ammonium ion in edible salt Chromatographic method co-eluting pairs Determination of nitric acid by cuvette method Chromatographic grade of nitric acid Imported chromatography Ion Chromatography Nitrate Peak Chromatographic grade nitric acid Ion Chromatography Hydrochloric Acid Chromatographic grade of nitric acid Chromatographic grade nitric acid Silicate Ion Chromatography Ion Chromatography in Food Chromatographic grade nitric acid Determination of Silicate by Ion Chromatography target ion colorimetry Silicate Ion Chromatography Nitrite chromatography method How to Automatically Integrate Ion Chromatography export food Determination method of sulfate ion chromatography Ion Chromatography Grade Nitric Acid Chromatographic separation material Chromatographic separation material Food exports to Central Asia Method for determination of nitrate in water Ion chromatography Dionex ion chromatography detection limit No peaks appear in ion chromatography Ion chromatography detection limit Method for detecting nitrite by ion chromatography Characteristics of the method for detecting chlorite using ion chromatography Ion chromatography detection limit Method for detecting sodium ions in feed National standard for detection method of sodium ions in feed Ion chromatography method detection limit Nitrate colorimetric method Determination of nitrate nitrogen by ion chromatography Detection limit of ion chromatography method National identification of ion chromatography Paper on detection limit of ion chromatography method Ion chromatography needle alignment method Biochromatography and affinity separation methods

Introduction

Analysis of Standard Technical Framework

Detection Method	Scope of Application	Detection Limit	Key Equipment
Colorimetry	Conventional Feed Samples	0.5 mg/kg	Spectrophotometer
Ion Chromatography	Complex Matrix Samples	0.1 mg/kg	Ion Chromatograph

Key points of core detection technology

Key steps of colorimetry

Use sulfanilic acid (C₆H₇NO₃S) to react with nitrite for diazotization
Add N-1-naphthylethylenediamine hydrochloride to generate a purple-red compound
Measure the absorbance at a wavelength of 538nm

Special requirements for ion chromatography

Requires AS19 chromatographic column (50mm×4mm)
Eluent gradient: 6-70mmol/L KOH solution
Injection volume: 250μL

Standard implementation recommendations

Sample pretreatment precautions

For high-protein feed (such as fish meal), it is recommended to use 70℃ water bath extraction for 15 minutes and then cool immediately to avoid protein interference. Liquid samples need to be filtered through a 0.22μm filter membrane.

Quality control requirements

Each batch of tests should include: blank control, spike recovery (80-110%) and parallel samples (RSD≤10%).

Technical development background

This standard replaces part of the GB/T 5009.33-2008 method. The main improvements include: reducing the detection limit by 50%, adding a solid phase extraction cleanup step, and introducing gradient elution chromatography technology.

More samples: JC/T 2453-2018 GB 50021-2001(2009) JGJ 94-2008

Sample only — not a preview of SN/T 5120-2019

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