SN/T 5145.11-2019 in English

VALID

Rapid ldentification of animal ingredient in export food and feed Part 11: Detection of Duck Ingredient PCR-Lateral flow dipstick method

Issued on:2019-10-25
Implemented on:2020-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $120.00
$117.00

Standard No:	SN/T 5145.11-2019
Document status:	VALID
Title in English:	Rapid ldentification of animal ingredient in export food and feed Part 11: Detection of Duck Ingredient PCR-Lateral flow dipstick method
Title in Chinese:	出口食品及饲料中动物源成分快速检测方法第11部分：鸭成分检测 PCR-试纸条法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-10-25
Implemented on:	2020-05-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	standard method detection sensitivity duck ingredient pcr-lateral flow dipstick method introduction interpretation standard technology technical parameters traditional method % detection time export feed company
Related Topics:	pcr detection strip pcr detection of fruit components Rapid detection of material composition Full component testing Animal Rapid Test Box Food Composition Detector Food and Beverage Testing Center Method Material Composition Testing Method Material Composition Testing Method pcr instrument food testing Animal composition ratio test Rapid detection of food of animal origin Feed Composition Detector Material Composition Detector pcr food testing and analysis What method is used to detect the composition of materials How to test for ingredients of animal origin Substance composition detection Substance composition detection Detection of material composition pcr food testing analysis pcr rapid detection products Phase Composition Detection Rapid detection method markers Rapid detection and detection analysis system Several methods of detecting ingredients tt Ingredient testing pcr components Checking Food Ingredients moisture detector Method for detection of substance composition test pcr pcr rapid detection kit PCR rapid detection device Hazardous substance testing\|safety testing\|electromagnetic compatibility testing\|physical reliability testing\|food contact material testing Drug ingredient testing PCR produces multiple strips Gas composition detection method Rapid detection method for fat detection in food Organic matter component detection method A rapid detection method for fiber in feed is Rapid moisture detection method in feed mills Film forming test method Flow rate detection method

Introduction

Interpretation of standard technology

Technical parameters	Traditional method	This standard method
Detection sensitivity	1-5%	0.1%
Detection time	24-72 hours	4-6 hours
Specificity	Cross-reaction rate>15%	<97% similarity

Core technology innovation

Using FITC labeled probe and biotin-streptavidin dual signal amplification system, through:

Specific primer pair (F:5'-TTCTACTACGGCTCCTACCTGTAT-3')
Optimized CTAB lysis buffer system (20g/L CTAB+1.4mol/L NaCl)
Three-step centrifugation purification process

Implementation points

Typical application case

In the detection of an export feed company, a DNA sample with an A260/A280 ratio of 1.82 was found. After pre-denaturation at 95℃, 30 cycles of amplification (94℃ 30s→55℃ 30s→72℃ 30s), the fluorescence threshold Ct value was 28.5, confirming that it was contaminated by duck-derived ingredients.

Analysis of standard evolution

Compared with the basic standard of GB/T 1.1-2009, this method:

Introduced lateral flow test strips for visual detection
Added genomic DNA purity determination formula: C=A×N×50/1000
Cleared negative/positive control setting requirements

More samples: YD/T 3934-2021 DL/T 368-2010 GB 50021-2001(2009)

Sample only — not a preview of SN/T 5145.11-2019

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