SN/T 5145.13-2019 in English

VALID

Rapid Identification of animal ingredient in export food and feed Part 13: Detection of dove Ingredient PCR-Lateral flow dipstick method

Issued on:2019-10-25
Implemented on:2020-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $120.00
$117.00

Standard No:	SN/T 5145.13-2019
Document status:	VALID
Title in English:	Rapid Identification of animal ingredient in export food and feed Part 13: Detection of dove Ingredient PCR-Lateral flow dipstick method
Title in Chinese:	出口食品及饲料中动物源成分快速检测方法第13部分：鸽子成分检测 PCR-试纸条法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-10-25
Implemented on:	2020-05-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	standard method sensitivity comparison pigeon ingredient detection microscope dove ingredient pcr-lateral flow dipstick method introduction interpretation method recovery rate technical standards test items traditional method % dna extraction phenol-chloroform method ctab
Related Topics:	pcr detection strip pcr detection of fruit components Rapid detection of material composition Full component testing Animal Rapid Test Box Food Composition Detector Food and Beverage Testing Center Method Material Composition Testing Method Material Composition Testing Method pcr instrument food testing Animal composition ratio test Rapid detection of food of animal origin Feed Composition Detector Material Composition Detector Rapid Molecular Detection pcr food testing and analysis What method is used to detect the composition of materials How to test for ingredients of animal origin Substance composition detection Substance composition detection Detection of material composition pcr food testing analysis pcr rapid detection products Phase Composition Detection Rapid detection method markers Polymer detection method Rapid detection and detection analysis system Several methods of detecting ingredients tt Ingredient testing pcr components Checking Food Ingredients moisture detector Method for detection of substance composition test pcr pcr rapid detection kit Hazardous substance testing\|safety testing\|electromagnetic compatibility testing\|physical reliability testing\|food contact material testing Drug ingredient testing PCR produces multiple strips Gas composition detection method Method for detecting sodium ions in feed National standard for detection method of sodium ions in feed Rapid detection method for fat detection in food Organic matter component detection method A rapid detection method for fiber in feed is Rapid moisture detection method in feed mills Molecular rapid test Flow rate detection method

Introduction

Interpretation of technical standards

Test items	Traditional method	This standard method	Sensitivity comparison
Pigeon ingredient detection	Microscope inspection/ELISA	Real-time fluorescence PCR	0.1% vs 1%
DNA extraction	Phenol-chloroform method	CTAB improved method	Recovery rate increased by 30%

Core technology innovation

Adopt FITC-biotin dual labeling system:
1. The 5' end of the forward primer is labeled with FITC fluorescein
2. The 5' end of the reverse primer is labeled with biotin
Signal amplification is achieved through the streptavidin-biotin system, and the detection limit is 0.1%

Key points for standard implementation

Sample pretreatment must comply with GB/T 14699.1 sampling specifications
DNA purity requires an A260/A280 ratio of 1.7-1.9
Amplification procedure: 94℃ pre-denaturation for 5min→30 cycles (94℃ 30s→55℃ 30s→72℃ 30s)→72℃ extension for 5min

Industry application cases

An export feed company adopts this standard:
1. Detect 0.5% pigeon-derived ingredients in mixed feed
2. Confirm sequence similarity> 97% through NCBI database comparison
3. Avoid EU notification incidents caused by adulteration of raw materials

More samples: DL/T 368-2010 NB/T 47013.2-2015 DL/T 5154-2012

Sample only — not a preview of SN/T 5145.13-2019

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