SN/T 5189-2020 in English
VALIDQuarantine protocol for Enteric septicemia of channel catfish
- Issued on:2020-12-30
- Implemented on:2021-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$262.00
| Standard No: | SN/T 5189-2020 |
| Document status: | VALID |
| Title in English: | Quarantine protocol for Enteric septicemia of channel catfish |
| Title in Chinese: | 鮰类肠败血症检疫技术规范 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2020-12-30 |
| Implemented on: | 2021-07-01 |
| Professional Classification: | SN-Import&Export Inspection |
| Related Keywords: | quarantine protocol
three-level quarantine enteric septicemia catfish introduction analysis standard technical framework |
| Related Topics: | intestinal
batch sepsis septicemia scattered disease Glutaremia anemia anemia failure syndrome blood coagulation Quarantine technology Catfish intestinal% "septicemia intestinal blood Detection Technology Type crystal violet staining Plasma indications |
Introduction
Analysis of the standard technical framework
This standard constructs a three-level quarantine technical system: clinical diagnosis → bacterial isolation and identification → molecular biology confirmation. The core detection indicators include:
| Detection dimensions | Traditional methods | PCR technology | Judgment criteria |
|---|---|---|---|
| Pathogen morphology | Gram-negative bacilli | N/A | Meet the characteristics of Appendix B |
| Biochemical characteristics | Glucose fermentation positive | N/A | 12 key indicators |
| Molecular markers | N/A | 276bp specific fragment | Sequence alignment ≥98% |
Key Technical Points
1. Clinical diagnostic features
Optical microscope observation should focus on:
- Acute type: intestinal congestion and edema, liver hemorrhagic necrosis
- Chronic type: skull perforation lesion diameter＞3mm
2. Laboratory testing process
- Bacterial isolation: Blood agar plate cultured at 28℃ for 48h
- Biochemical identification: motility test/oxidase test/OF test must be completed
- PCR detection: primer ESC-CM3/CM4 annealing temperature 55℃
Standard implementation recommendations
1. Sampling Specifications
According to SC/T 7103 requirements:
- Live sampling requires liver/kidney/brain composite samples
- Transport temperature maintained at 4-8℃
2. Key points of quality control
- ATCC 33202 positive control must be set for each batch of experiments
- PCR amplification cycle number is strictly controlled at 35cycle
Technology Evolution Analysis
Compared with the 2005 version of the standard, the main upgrades are:
- Added Eip gene-specific primers
- Optimized CTAB-DNA extraction process
- Clear sequencing verification requirements
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