SN/T 5202-2020 in English

VALID

Protocol for species identification of Cervus Nippon-Real-time PCR method

Issued on:2020-12-30
Implemented on:2021-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	SN/T 5202-2020
Document status:	VALID
Title in English:	Protocol for species identification of Cervus Nippon-Real-time PCR method
Title in Chinese:	梅花鹿物种鉴定技术规范—实时荧光PCR法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-12-30
Implemented on:	2021-07-01
Professional Classification:	SN-Import&Export Inspection
Related Topics:	fluorescent pcr PCR method pcr real time species Timed fluorescent quantitative PCR analysis Fluorescent PCR method fluorescent pcr Fluorescent PCR Multiplex Fluorescent PCR Fluorescence PCR instrument Real-time quantitative PCR analysis real-time fluorescence semi-quantitative Multiplex real-time quantitative PCR Timed Fluorometric Quantitative Method fluorescent signal Fluorescent real-time PCR Multiplex fluorescent real-time quantitative PCR Real-time PCR and multiplex PCR Multiplex real-time fluorescent quantitative PCR flow fluorescent pcr pcr method Q5 fluorescent quantitative PCR Fluorescent PCR+ Fluorescence real-time quantitative multiplex fluorescence Multiplex PCR Fluorescent Quantitative PCR Fluorescent quantitative PCR multiplex PCR Fluorescent quantitative PCR analysis method real-time fluorescence multiplex pcr vs fluorescent pcr Isothermal fluorescent PCR Isothermal fluorescent quantitative PCR Fluorescence Standard Fluorescence range real-time quantitative multiplex PCR Fluorescence PCR instrument Absolute Quantitative Fluorescent PCR Absolute Quantification by Fluorescent Quantitative PCR Fluorescence Multiplex fluorescent PCR technology multiplex fluorescent quantitative pcr technique Real-time fluorescent PCR Real-time fluorescent PCR instrument Fluorescence timed quantification real-time quantitative pcr multiplex relative real-time quantitative pcr pcr method pcr method Fluorescence range Fluorescence PCR instrument a relative fluorescence quantitative pcr relative fluorescence pcr real-time fluorescent quantitative pcr technology multiplex real-time fluorescent quantitative pcr real-time fluorescence quantitative PCR absolute quantification multiplex fluorescent quantitative pcr technique Multiplex PCR and Fluorescent Quantitative PCR Multiplex real-time fluorescent PCR instrument Multiplex PCR and fluorescent PCR Fluorescent PCR method Multiplex PCR real-time fluorescence quantitative PCR Relative Quantification by Fluorescent Quantitative PCR Fluorescence quantitative PCR relative quantification Real-time fluorescence quantitative PCR analysis Extremely fast fluorescence quantitative PCR fluorescent signal Fluorescence quantitative PCR Absolute quantification semi-quantitative real-time fluorescence fluorescent pcr peak Q5 real-time fluorescent quantitative PCR pcr fluorescence method Multiplex fluorescent PCR Real-time fluorescent PCR small real-time pcr absolute fluorescence quantitative pcr rna pcr method mini fluorescent pcr Real-time fluorescent PCR Q7 fluorescent quantitative PCR Real-time quantitative PCR, real-time fluorescence Q3 real-time fluorescent quantitative PCR femtosecond fluorescence technology Real-time fluorescent quantitative PCR peak shape analysis Biotechnology Fluorescence Analysis Semi-quantitative and real-time fluorescent quantitative PCR Fluorescence quantitative PCR peak species time multiple fluorescence technique ????pcr?? Real-time fluorescence quantitative PCR technology bioluminescent technology Q7 real-time fluorescent quantitative PCR Q7 real-time fluorescent quantitative PCR instrument Real-time fluorescent PCR instrument q5 Q7 fluorescent PCR Domestic fluorescence quantitative PCR PCR instrument fluorescence Fluorescent temperature How real-time fluorescence quantitative PCR is relatively quantitative Fluorescent quantitative PCR analysis method real-time fluorescence pcr technology application Q6 fluorescent quantitative PCR real-time fluorescence quantitative absolute Analytical method for real-time fluorescence quantification multiplex pcr fluorescence targeting pcr standard q6 fluorescent pcr species identification relative real-time fluorescent quantitative pcr Portable real-time fluorescence quantitative PCR instrument Fluorescent quantitative PCR peak species identification Flow Fluorescence Technology pcr instrument pcr technology application Triple fluorescent quantitative PCR Fluorescence PCR relative quantification Fluorescence data Real-time fluorescent quantitative PCR which PCR instrument Quantitative fluorescent PCR Ultra-high-speed real-time fluorescence quantification the time Real-time fluorescent PCR instrument Fluorescent quantitative PCR analysis method Quantitative Fluorescence Real-time PCR relative and absolute real-time fluorescence How long is real-time fluorescent quantitative PCR Q3 fluorescent quantitative PCR Fluorescent quantitative PCR analysis method Fluorescence quantitative PCR technology 5 items Real-time fluorescence quantification v7 Fluorescent quantitative PCR specificity Quantitative Fluorescence multistage pcr technology Real-time fluorescent quantitative PCR analysis Portable real-time fluorescent quantitative PCR Fluorescence real-time quantitative PCR Fluorescence quantitative PCR specificity real-time pcr analysis method multiplex fluorescent quantitative PCR Fluorescence quantitative + PCR + analysis Quantitative Time-PCR real-time fluorescent quantitative pcr and pcr Fluorescent real-time PCR real-time PCR Q5 fluorescent PCR Fluorescence sample preparation technology real-time fluorescence by pcr technology plum bossom real-time quantitative pcr Real-time fluorescent quantitative PCR peak Fluorescence quantitative and semi-quantitative PCR Several methods of real-time quantitative pcr Fluorescence PCR instrument usage method Fluorescent PCR cassette multiplex fluorescent PCR method Absolute Quantification and Relative Quantification of Real-time Fluorescence Quantitative PCR multiplex real-time fluorescence quantitative pcr technology Real-time fluorescent quantitative PCR instrument q5 Q5 real-time fluorescent quantitative PCR instrument q7 real-time fluorescent PCR instrument Fluorescent Antibody Technology fluorescence physics Real-time PCR instrument Real-time fluorescent Fluorescence quantification 2 pcr instrument pcr method Relative Quantification Real-time Fluorescence Quantification three-dimensional fluorescent technology Fluorescent Antibody Technology How to qualitative real-time fluorescent PCR Relative Quantification by Fluorescent Quantitative PCR flow real-time fluorescence Relative real-time fluorescence quantification Document Production Time Identification Technical Specification Identification pcr fluorescence method fluorescence physics Fluorescent timed quantitative PCR pcr+ method Fluorescence Signal Standard Relative Fluorescence Quantitative PCR and Absolute Fluorescence Quantitative PCR plate time fluorescence PCR initial fluorescence Real-time fluorescent PCR absolute quantification and relative quantification r2 of fluorescent quantitative pcr fluorescent pcr Real-time fluorescence semi-quantitative PCR pcr technique specific q6 real-time fluorescence The third generation PCR technology Fluorescent real-time quantitative PCR relative quantification fluorescent pcr iso fluorescence identification dna pcr technology pcr instrument Several pcr techniques real-time fluorescent quantitative pcr and pcr fluorescence real-time by pcr technique three kinds of pcr Real-time quantitative PCR analysis Fluorescent quantitative PCR technology per Fluorescence quantitative PCR technology in Isothermal PCR Real-time fluorescent PCR real-time fluorescence quantification relative quantification real-time quantitative technology pcr technology 35 pcr technology application pcr fluorescence method Various pcr techniques fluorescence The difference between digital PCR and fluorescence quantitative PCR What are the fluorescence quantitative PCR methods? Fluorescence quantitative PCR fluorescence intensity is low miRNA fluorescence quantitative PCR U6 Fluorescence quantification without technical repetition Fluorescence quantitative PCR has no ct value What is the difference between real-time quantitative PCR and real-time fluorescence quantitative PCR? rox fluorescent dye PCR optical module types Fluorescence staining identification

Introduction

Technical background of the standard

This standard replaces SN/T 3011.1-2011. Aiming at the species identification needs of products derived from sika deer (Cervus Nippon), it introduces real-time fluorescence PCR technology to improve detection sensitivity (up to 100±50 ng DNA) and specificity. Technological evolution is reflected in:

Core detection process

Steps	Key parameters	Technical points
DNA extraction	CTAB method	Proteinase K digestion + isopropanol precipitation
PCR system	20μL reaction volume	Contains UNG enzyme to prevent contamination
Cycling conditions	40 cycles	Annealing temperature 60℃

Result interpretation criteria

Positive judgment: When the Ct value is ≤35 and the control is normal, the sika deer component is detected. When the Ct value is in the range of 35-40, it needs to be retested for confirmation.

The typical amplification curve should show S-shaped characteristics, and the probe uses the FAM-TAMRA fluorescent labeling system.

Implementation recommendations

The laboratory should regularly verify the primer specificity (refer to Appendix A sequence)
It is recommended to set up triple controls (positive/negative/blank) for each batch of testing
For deep-processed samples, the DNA extraction plan needs to be optimized

More samples: GB 50021-2001(2009) DL/T 368-2010 YD/T 3934-2021

Sample only — not a preview of SN/T 5202-2020

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

SN 0283-1994 in English

\"Method for Inspection of Dichlorodipyridinol Residues in Exported Poultry Meat\"
1994-02-18
SN/T 0244-1994 in English

\"General Rules for Inspection and Certification of Imported Mechanical and Electrical Instruments\"
1993-08-01
SN 184-1993 in English

\"Test Methods for Listeria Monocytogenes in Exported Foods\"
1993-10-08
SN/T 5483.4-2022 in English

Protocol for assessing animal health status of exporting countryPart 4 :Bovine spongiform encephalopathy
2022-07-07