SN/T 5228.2-2019 in English

Technical background and significance of the standard

With the growth of international trade, the timeliness requirements for foodborne pathogen detection are increasing. The traditional culture method takes 5-7 days to detect Clostridium perfringens, while MALDI-TOF MS technology shortens the detection cycle to within 24 hours. As the second part of the SN/T 5228 series, this standard complements the GB 4789.13 method to meet the needs of rapid customs clearance of exported food.

Analysis of the core method

Key points of the detection process

Instrument parameter settings

• Detection mode: Linear positive ion mode
• Mass range: 2,000-20,000 Da
• Laser parameters: 60Hz frequency, 40 accumulations

Result judgment criteria

Implementation recommendations

1. Laboratories should establish a regular calibration system for MALDI-TOF MS
2. Pretreatment solutions should be optimized for high-fat samples
3. It is recommended to set up negative/positive controls for each batch of tests

Technology evolution analysis

Compared with the 2015 version of the industry standard, this method increases the detection sensitivity to 10³ CFU/mL, and for the first time clarifies the optimal conditions for anaerobic culture for 18-24h. In the future, it can form a methodological complement with whole genome sequencing technology.