SN/T 5367.1-2022 in English

VALID

Commercial kit method-Listeria monocytogenes - Test method I

Issued on:2022-03-14
Implemented on:2022-10-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $190.00
$185.00

Standard No:	SN/T 5367.1-2022
Document status:	VALID
Title in English:	Commercial kit method-Listeria monocytogenes - Test method I
Title in Chinese:	商品化试剂盒检测方法单核细胞增生李斯特氏菌方法一
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2022-03-14
Implemented on:	2022-10-01
Professional Classification:	SN-Import&Export Inspection
Related Keywords:	mds listeria monocytogenes molecular detection kit method-listeria monocytogenes genus listeria monocytogenes test method kit negative control
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Introduction

Interpretation of the core technology of the standard

This standard adopts the 3M MDS Listeria monocytogenes Molecular Detection Kit, which is based on the loop-mediated isothermal nucleic acid amplification (LAMP) technology and achieves specific detection by identifying 6 independent regions of the target sequence unique to the genus Listeria monocytogenes. The key technical features include:

Technical parameters	Traditional method (GB 4789.30)	LAMP method
Detection time	3-5 days	75 minutes
Sensitivity	1 CFU/25g	0.19 CFU/g
Equipment requirements	PCR instrument, etc.	Constant temperature equipment is sufficient

Key operation process

The test procedure is divided into three core stages:

Sample pretreatment: Use Demi-Fraser medium for 28-30 hours for primary enrichment, and cheese/fish samples require secondary enrichment with Fraser medium
DNA extraction: Lysis at 100℃ for 15 minutes, and obtain pink DNA template after cooling
LAMP amplification: Constant temperature reaction at 60℃, real-time fluorescence detection through the conversion of pyrophosphate to ATP

Quality control requirements

Typical application case: A dairy company used this method to detect positive samples, and the actual contamination level was confirmed to be 0.5 CFU/g by GB 4789.30, which was 72 hours earlier than the traditional culture method.

The standard clearly stipulates that double parallel controls must be set up:

Positive control: RC control is provided in the kit
Negative control: Sterile Demi-Fraser broth

Implementation suggestions

1. Laboratory partitioning: Strictly distinguish the sample processing area from the amplification analysis area to prevent aerosol contamination
2. Equipment validation: Regularly calibrate the temperature control system of the 3M molecular detector (60±1℃)
3. Consumables management: The lysis solution (LS tube) needs to be stored in the dark at 2-8℃
4. Result interpretation: The test result is valid only when both positive and negative controls meet expectations

More samples: JGJ 94-2008 GB 18306-2015 YD/T 3934-2021

Sample only — not a preview of SN/T 5367.1-2022

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