SN/T 5648-2024 in English
VALIDDetermination of benzovinflumizone residues in foods of animal origin - HPLC-MS/MS
- Issued on:2024-12-31
- Implemented on:2025-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$161.00
| Standard No: | SN/T 5648-2024 |
| Document status: | VALID |
| Title in English: | Determination of benzovinflumizone residues in foods of animal origin - HPLC-MS/MS |
| Title in Chinese: | 动物源食品中苯并烯氟菌唑残留量的测定 液相色谱-质谱/质谱法 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2024-12-31 |
| Implemented on: | 2025-07-01 |
| ICS Classification: | 67.050-General methods of tests and analysis for food products |
| Chinese Classification: | C53-Food sanitation |
| Professional Classification: | SN-Import&Export Inspection |
| Related Keywords: | benzovinflumizone residues
benzovindiflupyr residues animal-derived foods hplc-ms/ms introduction technical background detection method |
Introduction
Technical background and evolution of the standard
As the first industry standard for the detection of benzovindiflupyr residues in animal-derived foods in my country's customs system, this standard fills the gap in the detection method of this fungicide in livestock, poultry and aquatic products. Compared with EU Regulation EU 2017/625, it innovatively includes special matrices such as propolis in the detection range, and the quantitative limit reaches the international advanced level (5.0μg/kg).
Principle and technical characteristics of the method
| Technical elements | This standard protocol | QuEChERS traditional method |
|---|---|---|
| Extraction solvent | 1% acidic acetonitrile (pure acetonitrile for propolis) | Acetonitrile-water system |
| Purification method | Phospholipid-removed PPR column (200mg/3mL) | PSA+C18 dispersed purification |
| Mass spectrometry conditions | MRM mode monitoring 3 pairs of ions (322.1*quantitative) | Usually two pairs of ions are monitored |
Analysis of key operation points
Differences in sample pretreatment
Different treatments are used for different matrices: propolis needs to be frozen and crushed at -20℃ and then extracted with acetonitrile, while pig fat extract needs to be frozen and defatted at -18℃. Special treatment stabilizes the recovery rate between 81.5-113.0% (see Table B.1).
Optimization of chromatographic conditions
A C18 column (2.6μm) was used with a gradient elution of 5mmol/L ammonium formate-0.1% formic acid aqueous solution/methanol to stabilize the retention time at 4.2±0.1min, effectively separating matrix interference.
Implementation recommendations
- Quality control: A blank matrix spike experiment should be performed for each batch of samples, and the recovery rate should meet the requirements of Appendix B
- Instrument maintenance: The ESI source lens needs to be cleaned every week to prevent formic acid residue from causing a decrease in sensitivity
- Method validation: The limit of detection (LOD) should be validated when used for the first time in the laboratory, and it is recommended to be 1/3 of the limit of quantification
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
SN/T 5075-2018 in English
Specification for sample preparation for microbiological examination of cosmetics
2018-03-12 -
SN/T 3628-2013 in English
Determination of 11 dinitroaniline herbicide residues in botanical foodstuffs for export.GC-MS/MS method
2013-08-30 -
SN/T 5654-2024 in English
Detection of porcine origin components in gelatin by liquid chromatography-tandem mass spectrometry
2024-12-31 -
SN/T 5325.6-2020 in English
Digital PCR method for quantitative detection of foodborne viruses in exported foods Part 6: Coxsackie virus
2020-12-30
