WS/T 776-2021 in English
VALIDSpecification for environmental monitoring of SARS-CoV-2 in agriculture product markets and trade markets
- Issued on:2021-02-20
- Implemented on:2021-02-20
- File Format:PDF
- Delivery:Via email within 1~3 business days
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本文件规定了新型冠状病毒肺炎疫情防控中，对农贸（集贸）市场等场所环境和从业人员开展新型冠状病毒监测的监测对象、监测内容、监测方法及实验室生物安全的要求。
本文件适用于新冠肺炎疫情流行和常态化防控期间，疫情防控相关机构和人员对农贸（集贸）市场等场所环境和从业人员开展监测，包括现场采样、实验室检测等内容。
Introduction
Interpretation of the core content of the standard
This standard establishes a systematic environmental monitoring system for the new coronavirus for high-risk places such as farmers' trade collective markets. Technical points include:
Hierarchical management of monitoring objects
| Monitoring type | Key objects | Monitoring frequency |
|---|---|---|
| Environmental monitoring | Cold chain stalls, poorly ventilated areas | 1-2 times/week |
| Personnel monitoring | Employees in high-risk stalls | According to the requirements of the prevention and control plan |
Innovative sampling technology
The standard proposes the multi-point distributed sampling method for the first time: dividing the surface of large objects into 3-5 10cm² sampling areas, which significantly improves the virus detection rate. Specific application scenarios include:
- Surface sampling of large cold chain equipment
- Monitoring of countertops at wholesale stalls
- Multi-point sampling of transportation vehicles
Laboratory testing specifications
Wastewater sample treatment case
A seafood market test uses the 4654g centrifugation + ultrafiltration concentration technical process:
- 4℃ low-temperature centrifugation for 30 minutes to remove impurities
- Use 10-100kDa ultrafiltration tube for concentration
- Finally obtain 0.5-2mL of test solution
Biosafety requirements
| Operation links | Protection level | Critical control points |
|---|---|---|
| Sample packaging | BSL-2+Level 3 protection | Operation in a biosafety cabinet |
| Sample transportation | UN3373 packaging | Transfer by dedicated personnel and vehicle within 24 hours |
Implementation suggestions
It is recommended to establish a three-level monitoring network:
- Market self-inspection: daily rapid testing in key areas
- Routine disease control monitoring: Execute at standard frequency
- Emergency monitoring: encrypt monitoring during epidemic response
At the same time, professional equipment such as aerosol samplers should be equipped, and the sampling height should be strictly controlled within the range of 1.2-1.5m.
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