GA/T 1913-2021 in English
VALIDForensic sciences-Examination methods for gelsemine and koumine in biological samples-LC-MS
- Issued on:2021-10-14
- Implemented on:2022-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
Price(USD)：
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$127.00
$127.00
Introduction
Analysis of Standard Technical Framework
| Technical Elements | Liquid-Liquid Extraction | Solid Phase Extraction |
|---|---|---|
| Applicable Samples | Blood/Urine/Tissue Homogenate | Complex Matrix Samples |
| Recovery Rate | 85-92% | ≥95% |
| Purification Effect | Medium | Excellent |
Key Instrument Parameter Settings
Special attention should be paid when using liquid chromatography-mass spectrometer:
- Chromatographic column:Kinetex C18 (50×3.0mm,2.6μm)
- Ion source:ESI+ mode, voltage 5500V
- Characteristic ion pairs:Herceptin A 323.2>236.1/195.2, Herceptin 307.2>180.2/167.0
Key points for implementation of the standard
Quality control requirements
Blank samples and spiked samples (20ng/mL concentration) must be operated in parallel, and the relative difference is ≤20% to determine the data to be valid. Actual cases show that special attention should be paid to matrix effect interference in liver tissue samples.
Method Validation Data
| Indicators | Collinin A | Collinin B |
|---|---|---|
| Detection Limit | 1ng/g | 1ng/g |
| Linear Range | 1-200ng/mL | 1-200ng/mL |
Technology Evolution Analysis
Compared with the early GC-MS method, this standard LC-MS method has three major advantages:
- Avoiding alkaloid decomposition caused by high temperature
- MRM mode improves detection specificity
- The detection limit is reduced by more than 10 times
Sample only — not a preview of GA/T 1913-2021
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