GB/T 14702-2018 in English

《GB/T 14702-2018添加剂预混合饲料中维生素B6的测定 高效液相色谱法》由TC76（全国饲料工业标准化技术委员会）归口，主管部门为国家标准化管理委员会。

1 Scope

This standard specifies the method for determination of vitamin B6 content in feeds by high-performance liquid chromatography.

This standard is applicable to the determination of vitamin B6 in compound premix and vitamin premix with vitamin B6 content greater than 30 mg/kg.

2 Normative References

The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this part. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.

GB/T 6682 Water for Laboratory Use — Specifications and Test Methods
GB/T 14699.1 Animal Feeding Stuffs — Sampling

3 Principle

After ultrasonic extraction of the acid extract of vitamin B6 specimen, the test solution is injected into a high-performance liquid chromatography (HPLC) system for separation. Ultraviolet (or diode matrix detector) is used for detection. The content of vitamin B6 can be calculated by external standard method.

4 Reagent and solution

Unless otherwise specified, the reagents used shall be of analytical grade, the water used shall be distilled water, and the deionized water for chromatographic use shall meet the requirements of Grade 1 water specified in GB/T 6682.1.

Foreword II
1 Scope
2 Normative References
3 Principle
4 Reagent and solution
5 Apparatus
6 Preparation of Specimen
7 Analytical Procedure
8 Calculation of Results
9 Repeatability