GB/T 17817-2024 in English
VALIDDetermination of vitamin A in feeds—High performance liquid chromatography
- Issued on:2024-11-28
- Implemented on:2025-06-01
- File Format:PDF
- Delivery:Via email within 5 business days
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《GB/T 17817-2024饲料中维生素A的测定 高效液相色谱法》由TC76（全国饲料工业标准化技术委员会）归口，主管部门为国家标准委。
Introduction
Interpretation of the Standard for the Determination of Vitamin A in Feed by High Performance Liquid Chromatography
This standard (GB/T 17817-2024) specifies the high performance liquid chromatography method for the determination of vitamin A in feed, replacing the old standard (GB/T 17817-2010). This article will provide a comprehensive interpretation of the standard from the aspects of standard background, technical specifications, implementation recommendations, etc.
1. Background of Standard Formulation
Vitamin A is an important nutrient in animal feed, and its content directly affects the health and growth performance of animals. With the development of the feed industry, higher requirements are put forward for the detection of vitamin A. This standard has made a number of improvements on the basis of the old version, including:
- Increase the scope of application of concentrate supplements and compound premixed feeds
- Optimize the extraction agent to be petroleum ether (boiling range 30 ℃ ~ 60 ℃)
- Add offline solid phase extraction method and online solid phase extraction method
- Improve the precision of the limit of quantification, the limit of quantification of the first method is 1 000 IU/kg, and the limit of quantification of the second method is 20 000 IU/kg
2. Comparison of standard frameworks
| Dimensions | Old version of the standard (GB/T 17817-2010) | New version of the standard (GB/T 17817—2024) |
|---|---|---|
| Scope of application | Mainly suitable for compound feed and concentrated feed | Add concentrate supplement and compound premix feed |
| Extraction method | Single liquid-liquid extraction method | Newly added offline solid phase extraction method and online solid phase extraction method |
| Limit of quantification | The limit of quantification of the first method is 500 IU/kg | The limit of quantification of the first method is increased to 1 000 IU/kg, and that of the second method is increased to 20 000 IU/kg |
3. Method interpretation and technical specifications
3.1 Method 1: Saponification extraction method
Principle:After the sample is saponified with an alkaline solution, it is purified by liquid-liquid extraction or solid phase extraction, and separated and determined by high performance liquid chromatography.
Scope of application:Determination of vitamin A in compound feed, concentrate supplement, concentrated feed, compound premix feed, and vitamin premix feed.
Actual application case
A feed factory uses saponification extraction to detect the content of vitamin A in compound feed. The results show that this method has high accuracy and sensitivity.
3.2 Second method: direct extraction method
Principle:Directly extract vitamin A acetate in the sample with methanol and determine it by high performance liquid chromatography.
Scope of application:Determination of vitamin A acetate in compound premix feed and vitamin premix feed.
4. Implementation Suggestions
To ensure accurate test results, it is recommended that:
- Operate in strict accordance with the reagent ratios and instrument parameters specified in the standards
- Keep samples away from light during preparation
- Choose a suitable chromatographic column and mobile phase combination
- Calibrate instruments and equipment regularly
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