GB/T 32016-2015 in English

《GB/T 32016-2015蚕丝 氨基酸的测定》由TC401（全国丝绸标准化技术委员会）归口，主管部门为中国纺织工业联合会。
本标准规定了蚕丝中氨基酸成分及含量的分析测定方法。
本标准适用于蚕丝中十八种氨基酸组分的测定。天冬氨酸(Asp)、苏氨酸(Thr)、丝氨酸(Ser)、谷氨酸(Glu)、甘氨酸(Gly)、丙氨酸(Ala)、缬氨酸(Va1)、异亮氨酸(Ile)、亮氨酸(Leu)、酪氨酸(Tyr)、苯丙氨酸(Phe)、赖氨酸(Lys)、组 氨 酸 (His)、精 氨 酸 (Arg)、脯 氨 酸 (Pro)采 用 酸 水 解 法 测 定,胱 氨 酸(Cys)和蛋氨酸(Met)若采用酸水解法不能准确测定、应采用氧化水解法测定,色氨酸(Trp)采用碱水解法测定。其最低检出限均为10pmol。

Scope

This standard specifies the analysis and determination methods of amino acid composition and content in silk. This standard applies to the determination of eighteen kinds of amino acid components in silk. Aspartic acid (Asp), threonine (Thr), serine (Ser), glutamic acid (Glu), glycine (Gly), alanine (Ala), valine (Val), isoleucine (Ile), Leucine (Leu), Tyrosine (Tyr), Phenylalanine (Phe), Lysine (Lys), Histidine (His), Arginine (Arg), Proline (Pro) Determination by acid hydrolysis method. If cystine (Cys) and methionine (Met) cannot be accurately determined by acid hydrolysis method, oxidative hydrolysis method should be used for determination. Tryptophan (Trp) should be determined by alkaline hydrolysis method. The minimum detection limit is 10 pmol.