GB/T 44214-2024 in English
VALIDTest method for non-protein content in silk
- Issued on:2024-07-24
- Implemented on:2025-02-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$262.00
《GB/T 44214-2024蚕丝中非蚕丝蛋白物质含量试验方法》由TC401(全国丝绸标准化技术委员会)归口,主管部门为中国纺织工业联合会。
Introduction
Explanation of the test method
This standard is applicable to the calculation of the content of non-silk protein substances in silk by determining the content of four main amino acids (serine, glycine, alanine and tyrosine) in silk.
| Item | Mulberry silk | Tussah silk | Test method |
|---|---|---|---|
| Sample mass | 1g | 1g | GB/T 9995 |
| Hydrolysis time | 24h | 48h | 110°C ± 2°C |
| Amino acid determination | HPLC method | HPLC method | Appendix A |
Comparative analysis of standard frameworks
This standard is in line with the internationally accepted textile testing methods. The following are the main comparison dimensions:
- Scope of application:Applicable only to silk and its products
- Determination principle:Amino acid pre-column derivatization HPLC method
- Precision requirement:Absolute difference of parallel samples ≤5% of the arithmetic mean
Explanation of professional terms
Non-silk protein substances refer to substances that are not decomposed into amino acids after silk is hydrolyzed, mainly including abnormally free NH₄⁺ and grafted polymers.
Actual application case
Test results of a batch of mulberry silk samples:
- Serine content:5.2%
- Glycine content:4.8%
- Alanine content:6.3%
- Tyrosine content:12.0%

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