GB/T 34739-2017 in English
VALIDDetecting technique of neutralization antibody against rabies virus in animals
- Issued on:-
- Implemented on:-
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 34739-2017动物狂犬病病毒中和抗体检测技术》由TC181（全国动物卫生标准化技术委员会）归口，主管部门为农业农村部。
Introduction
1. Background and significance of standard formulation
GB/T 34739-2017 "Detection technology of neutralizing antibodies to animal rabies virus" is a standard formulated in accordance with the relevant requirements of the International Terrestrial Animal Health Code (OIE) and in combination with my country's actual situation. This standard is mainly used to evaluate the immune protection of animals against rabies, and provide a scientific basis for vaccine development, immune effect monitoring and epidemiological research.
2. Overview of FAVN detection method
FAVN (Fluorescent Antibody Virus Neutralization Test) is a virus neutralization test based on fluorescent antibodies, which is used to quantitatively determine rabies virus neutralizing antibodies in animal serum. This method has high sensitivity and high specificity and is widely used in veterinary laboratories.
3. Analysis of the test operation process
| Steps | Specific requirements | Key points |
|---|---|---|
| Sample collection | Aseptically collect ≥2mL blood and let stand at 4℃ for more than 1 hour. | Make sure to record animal information to avoid contamination. |
| Serum treatment | Inactivate in a 56℃ water bath for 30 minutes and centrifuge at 4000r/min for 10 minutes. | Use secondary pure water to prepare the solution. |
| Virus titration | BHK-21 cell culture, determination of CVS-11 strain TCID50 value ≥10^5 TCID50/mL. | The operation must be carried out in a BSL-2 laboratory. |
4. Experimental materials and equipment requirements
Reagent requirements:Including analytical reagents, fluorescent labeled antibodies (FITC), cell culture medium (MEM), etc.
Equipment requirements:Equipped with professional instruments such as CO₂ incubator, inverted fluorescence microscope, 8-channel pipette, etc.
5. Comparative analysis of standard frameworks
| Standard dimensions | GB/T 34739—2017 | OIE recommended method | Difference analysis |
|---|---|---|---|
| Detection principle | FAVN method | FAVN method | Completely consistent |
| Scope of application | Animal individual and group immunity assessment | Mainly used in international trade | Wider application |
6. Implementation suggestions
Laboratory construction:Constructed strictly in accordance with BSL-2 standards, equipped with professional equipment and protective facilities.
Staff training:Operators must complete rabies pre-exposure immunization and regularly test antibody titers.
Quality control:Establish a standardized quality control system to ensure the stability of reagents, cell lines and virus strains.
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
GB/T 17494-2023 in English
Diagnostic techniques for equine infectious anemia
2023-09-07 -
GB/T 44613-2024 in English
Diagnostic techniques for bovine and caprine parainfluenza virus type 3
2024-09-29 -
GB/T 27980-2011 in English
Diagnostic techniques for equine viral arteritis
2011-12-30 -
GB/T 27621-2024 in English
Diagnostic techniques for equine rhinopneumonitis
2024-11-28 -
GB/T 16550-2020 in English
Diagnostic techniques for newcastle disease
2020-12-14 -
GB/T 42070-2022 in English
Treatment of piglets diarrhea based on syndrome differentiation in traditional Chinese veterinary medicine
2022-10-12 -
GB/T 42304-2023 in English
Welfare criteria for animals to be slaughtered
2023-03-17 -
GB/T 45100-2024 in English
Diagnostic techniques for enzootic abortion of ewes
2024-12-31 -
GB/T 47130-2026 in English
Diagnostic techniques for bovine coronavirus infection
2026-01-28
