GB/T 34797-2017 in English

VALID

Quality technical requirements of primers and probes

Issued on:2017-11-01
Implemented on:2018-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $220.00
$214.00

Standard No:	GB/T 34797-2017
Document status:	VALID
Title in English:	Quality technical requirements of primers and probes
Title in Chinese:	核酸引物探针质量技术要求
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-11-01
Implemented on:	2018-05-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	G66-Biochemical reagent and clinical analysis reagent
Professional Classification:	GB-National Standard
Related Keywords:	quality requirements standard frameworks standard dimensions primer requirements probe requirements technical requirements storage requirements dna primer probes
Related Topics:	Primer Primer quality Probe pyrene Nucleic acid primer probe quality technical requirements u6 probe Primer quality microprobe technology Probe primer specificity Probe primer p2 primer primer probe probe membrane Primer + Batch Nucleic Acid Primer Probe Quality Technology Primer quality Angstrom Primer requirements GB/T 34797 southern probe Forward primer and upstream primer primer probe Primer for u6 probe carbon Probe Minerals silver probe probe technology probe technology Kelvin probe sample preparation requirements Primer probe quality technical requirements gb34797 Nucleic acid probe primer quality requirements negative probe Frontiers of Nucleic Acid Technology forward primer primer Probe series Kelvin Probe Technology Novel Chemical Probe Technology hemicyanide probe dose probe Primer content three-primer primer Probe series three-primer method two-primer method probe density tank probe Probe station probe Peptide probe Test Probe Requirements v6 probe Material detection method nucleic acid probe acid peak u6 primer primer Probe Components Probe Test Technology random primer Nucleic acid probe single strand nucleic acid hybridization probe matter probe water quality probe Peptide Nucleic Acid Probe Hybridization target probe Peptide Nucleic Acid Probe Hybridization probe measurement technique Nucleic acid quantitative machine Nucleic Acid Analysis Technology random primer quality water quality probe Random Primer + Quality pyrene probe Probe Measurement System upstream primer primer probe antenna v5 probe Isothermal Primer Requirements probe action Probe station probe GB/T 34797-2017 Kelvin Probe Technology probe antenna upstream primer forward primer Test Probe Requirements Probe Probe Holder Nucleic acid system dual probe technique pcr nucleic acid technology hollow probe low probe Rare Earth Probe Probe Sheet Carbon nucleic acid probe Mechanical probe measurement low-dimensional probe positioning probe nucleic acid probe Probes for Nucleic Acid Hybridization perylene probe GBT34797 GB/T 34797-2017 Sample requirements for electronic probes Nuclear Detection Technology Laboratory Reduce nucleic acid primer solubility Principles of primer design for probe method Primer and probe design principles Lead quality of pt100 Nucleic acid probe labeling Probe piece sprayed with carbon NCBI design quantitative primers SNOM probe Taqman probe primer design principles Probe annotation Air conditioning quality requirements technology protein probe Nucleic acid probe labeling method Geophysical equipment technical standards Labeling methods for nucleic acid probes Bed of Nails Test Probes

《GB/T 34797-2017核酸引物探针质量技术要求》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

1. Standard Overview

GB/T 34797-2017 "Technical Requirements for Quality of Nucleic Acid Primers and Probes" specifies the quality evaluation indicators, technical requirements, test methods, packaging, transportation and storage requirements of DNA primer probes. This standard applies to the quality evaluation and detection of oligonucleotide products such as DNA primer probes.

2. Background of Standard Formulation

With the development of biotechnology, especially the widespread application of gene detection and PCR amplification technology, the quality requirements of nucleic acid primer probes are becoming increasingly important. This standard aims to ensure the reliability and consistency of primers and probes in scientific research and clinical applications.

3. Comparison of standard frameworks

Standard dimensions	Primer requirements	Probe requirements
Total OD	≤10%	10%
Base accuracy	Consistent with custom sequence	Consistent with custom probe sequence
Purity level	Desalting grade (≥75%) to HPLC grade (≥95%)	HPLC grade (≥95%)

4. Interpretation of technical requirements

According to the standards, the quality control indicators of primers and probes include total OD, base accuracy, purity level, base deletion rate, relative molecular mass, etc. For example, HPLC-grade purity primers require a purity of ≥95%, while probes only need to meet HPLC-grade purity (≥95%). In addition, the relative error of relative molecular mass is required to be ≤0.05%.

5. Implementation suggestions

5.1 Quality control process

Use first-grade water for experiments in accordance with GB/T 6682.
Use mass spectrometry or high-performance liquid chromatography to determine purity and relative molecular mass.
Verify the specificity and effect of primers and probes through PCR amplification experiments.

5.2 Precautions

The synthesis report should include information such as sequence, length, purification method, and modification group.
Storage conditions must be strictly followed to avoid repeated freezing and thawing that may affect product quality.
Prevent heavy pressure and sunlight during transportation to ensure safe delivery of the product.

More samples: YD/T 3934-2021 NB/T 47013.2-2015 DL/T 5154-2012

Sample only — not a preview of GB/T 34797-2017