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standard overview ribonuclease a standard frameworks standard dimensions technical requirements test methods scope facan unit/mg protein uv spectrophotometer method high purity requirements impurity detection shall off-white powder visual inspection method all product batches enzyme activity standard requirements mineral processing flexible guide rails production management documents
GB/T 35543-2017 in English

GB/T 35543-2017 in English

VALID

Ribonuclease A

  • Issued on:2017-12-29
  • Implemented on:2018-07-01
  • File Format:PDF
  • Delivery:Via email within 1~3 business days
Price(USD): $160.00
$156.00
Standard No: GB/T 35543-2017
Document status: VALID
Title in English: Ribonuclease A
Title in Chinese: 核糖核酸酶A
Language: English
File Format: Electronic (PDF)
Delivery: Via email within 1~3 business days
Issued on: 2017-12-29
Implemented on: 2018-07-01
ICS Classification: 07.080-Biology. Botany. Zoology
Chinese Classification: C27-Biological agent and blood products
Professional Classification: GB-National Standard
Related Keywords: standard overview ribonuclease a
standard frameworks standard dimensions technical requirements test methods scope
facan unit/mg protein uv spectrophotometer method high purity requirements impurity detection shall
off-white powder visual inspection method all product batches enzyme activity
standard requirements
Related Topics: enzyme
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ribonucleic acid
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GBT35543
GB/T 35543-2017
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《GB/T 35543-2017核糖核酸酶A》由SWG11(全国工具酶标准化工作组)归口,主管部门为国家标准化管理委员会。


Introduction

1. Standard Overview

Ribonuclease A is an endonuclease derived from bovine pancreas and is widely used in molecular biology, medicine and biotechnology. The GB/T 35543-2017 standard specifies its technical requirements, test methods and storage conditions, providing standardized guidance for production and use.

2. Comparison of standard frameworks

Standard dimensions Technical requirements Test methods Scope of application
Appearance White or off-white powder Visual inspection method All product batches
Enzyme activity (specific activity) ≥3500 Facan unit/mg protein UV spectrophotometer method High purity requirements
Impurity detection Shall not contain other endonucleases and exonucleases Plasmid electrophoresis Production quality control

3. Background of standard formulation and technological evolution

With the development of biotechnology, ribonuclease A is increasingly used in gene editing and molecular diagnosis. The formulation of this standard aims to promote the industrialization of tool enzymes and ensure product quality and consistency.

4. Implementation suggestions

  • Production process: strictly control the source of raw materials and extraction process to ensure product purity and activity.
  • Inspection process: establish a complete quality control system and calibrate the detection instruments regularly.
  • Storage conditions: store below -20°C in accordance with standard requirements to avoid enzyme activity loss.

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