GB/T 35543-2017 in English
VALIDRibonuclease A
- Issued on:2017-12-29
- Implemented on:2018-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$156.00
《GB/T 35543-2017核糖核酸酶A》由SWG11(全国工具酶标准化工作组)归口,主管部门为国家标准化管理委员会。
Introduction
1. Standard Overview
Ribonuclease A is an endonuclease derived from bovine pancreas and is widely used in molecular biology, medicine and biotechnology. The GB/T 35543-2017 standard specifies its technical requirements, test methods and storage conditions, providing standardized guidance for production and use.
2. Comparison of standard frameworks
| Standard dimensions | Technical requirements | Test methods | Scope of application |
|---|---|---|---|
| Appearance | White or off-white powder | Visual inspection method | All product batches |
| Enzyme activity (specific activity) | ≥3500 Facan unit/mg protein | UV spectrophotometer method | High purity requirements |
| Impurity detection | Shall not contain other endonucleases and exonucleases | Plasmid electrophoresis | Production quality control |
3. Background of standard formulation and technological evolution
With the development of biotechnology, ribonuclease A is increasingly used in gene editing and molecular diagnosis. The formulation of this standard aims to promote the industrialization of tool enzymes and ensure product quality and consistency.
4. Implementation suggestions
- Production process: strictly control the source of raw materials and extraction process to ensure product purity and activity.
- Inspection process: establish a complete quality control system and calibrate the detection instruments regularly.
- Storage conditions: store below -20°C in accordance with standard requirements to avoid enzyme activity loss.

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