GB/T 35904-2018 in English

VALID

Real-time PCR method for detection of Trichinella spiralis

Issued on:2018-02-06
Implemented on:2018-09-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $100.00
$97.00

Standard No:	GB/T 35904-2018
Document status:	VALID
Title in English:	Real-time PCR method for detection of Trichinella spiralis
Title in Chinese:	旋毛虫实时荧光PCR检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-02-06
Implemented on:	2018-09-01
ICS Classification:	11.220-Veterinary medicine
Chinese Classification:	B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification:	GB-National Standard
Related Keywords:	real-time fluorescence pcr detection method real-time pcr method trichinella spiralis real-time fluorescence pcr technology technical evolution trichinella spiralis infection
Related Topics:	fluorescent pcr pcr Fluorescence detection Used Fluorescence Detectors fluorescent pcr Isothermal fluorescent PCR instrument Fluorescent PCR Multiplex Fluorescent PCR Optical rotation experiment Fluorescent quantitative multiplex PCR detection Fluorescent PCR and multiplex Fluorescence detection standard pcr detection unit Real-time quantitative PCR analysis pcr+ real-time fluorescence semi-quantitative Multiplex real-time quantitative PCR Fluorescence detection Timed Fluorometric Quantitative Method In vitro fluorescence detection Multiplex fluorescent quantitative PCR detection flow fluorescence and pcr Fluorescence detection equipment Fluorescent Material Detection optical method Multiplex fluorescent real-time quantitative PCR multiplex fluorescent quantitative pcr method Real-time PCR and multiplex PCR Detection of fluorescent peaks Multiplex real-time fluorescent quantitative PCR flow fluorescent pcr What are the second-hand fluorescence detectors? Q5 fluorescent quantitative PCR Fluorescent PCR+ Fluorescence real-time quantitative multiplex fluorescence Multiplex PCR Fluorescent Quantitative PCR Fluorescent quantitative PCR multiplex PCR Multiplex fluorescent PCR enzyme Fluorescent quantitative PCR analysis method real-time fluorescence Real-time fluorescent PCR detection method for Borrelia burgdorferi at border ports multiplex pcr vs fluorescent pcr Isothermal fluorescent PCR Isothermal fluorescent quantitative PCR Fluorescent PCR detection real-time quantitative multiplex PCR how to use pcr Absolute Quantitative Fluorescent PCR fluorescent pcr gelatin Real-time fluorescent PCR Multiplex real-time PCR analysis method multiplex fluorescent quantitative pcr real-time quantitative pcr multiplex relative real-time quantitative pcr Optical rotation detection Measurement of specific rotation by fluorescence method Fluorescence detector peak On-line fluorescence detection pcr method pcr method Time Fluorescent Test Strips Optical rotation detection relative fluorescence quantitative pcr Q5 fluorescent PCR instrument pcr secondary pcr relative fluorescence pcr Isothermal fluorescent PCR instrument multiplex real-time fluorescent quantitative pcr Fluorescence performance testing real-time fluorescence quantitative PCR absolute quantification dna pcr test Fluorescence online detection Real-time PCR analysis Multiplex PCR and Fluorescent Quantitative PCR Multiplex real-time fluorescent PCR instrument Multiplex PCR and fluorescent PCR Real-time fluorescence quantitative PCR instrument q3 Fluorescent PCR detection multiplex fluorescent pcr method Optical rotation detection method liquid detection fluorescence temporal fluorescence peak Fluorescent German Fluorescent quantitative PCR detection method for Bacillus anthracis Multiplex PCR real-time fluorescence quantitative PCR Detection by PCR, multiplex PCR and fluorescent quantitative PCR Real-time fluorescence quantitative PCR analysis In vitro fluorescence detection system Real-time fluorescent PCR detection method for Trichinella spiralis Extremely fast fluorescence quantitative PCR Fluorescence detection method Fluorescence detection method Fluorescence Detector Premium Fluorescence detection method by flow cytometry semi-quantitative real-time fluorescence Fluorescent Material Detection Liquid fluorescence test method How to use the PCR instrument fluorescent pcr peak Fluorescence time curve Q5 real-time fluorescent quantitative PCR Multiplex PCR Fluorescent real-time PCR Quantitative PCR multiplex fluorescence detection Fluorescence performance testing Multiplex fluorescent PCR Real-time fluorescent PCR small real-time pcr Fluorescence detection led Column fluorescence detection method Optical Spectroscopy absolute fluorescence quantitative pcr rna pcr method Real-time quantitative fluorescence for food detection mini fluorescent pcr Real-time fluorescent PCR Q7 fluorescent quantitative PCR Real-time quantitative PCR, real-time fluorescence multiplex pcr several fluorescence Q3 real-time fluorescent quantitative PCR chicken fluorescent pcr real-time fluorescence detection When detecting fluorescence spectra? Real-time fluorescent quantitative PCR peak shape analysis polarimetry Fluorescence detector cell Fluorescence detector 1 Semi-quantitative and real-time fluorescent quantitative PCR Fluorescence quantitative PCR peak when pcr upstream primer Optical rotation detector Q7 real-time fluorescent quantitative PCR Fluorescence Detector Detector Q7 real-time fluorescent quantitative PCR instrument Real-time fluorescent PCR instrument q5 Q7 fluorescent PCR Real-time detector Domestic fluorescence quantitative PCR isothermal fluorescence detection Fluorescent temperature pcr power How to use pcr machine Liquid Fluorescence Test Method Fluorescent quantitative PCR analysis method real-time fluorescence Q6 fluorescent quantitative PCR quadratic pcr Definition of real-time detection Fluorescent detection of serum Fluorescence detection food detection Trichinella detection Detection of autofluorescence real-time fluorescence quantitative absolute Analytical method for real-time fluorescence quantification autofluorescence detection Fluorescent method multiplex pcr fluorescence targeting Ordinary PCR and fluorescent PCR q6 fluorescent pcr Fluorescence food detection fluorescence method fluorescence time pcr rna detection relative real-time fluorescent quantitative pcr mir pcr detection polarimetry Light source detection method Fluorescence detection plate Fluorescent PCR and Fluorescent Probes Portable real-time fluorescence quantitative PCR instrument Fluorescent quantitative PCR peak Fluorescence detector series Molecular fluorescence detection Fluorescence detection and UV detection Optical rotation detector Fluorescent PCR and common PCR Fluorescence PCR and common PCR pcr relative quantification method Concentration when measuring fluorescence pcr relative quantification method Triple fluorescent quantitative PCR Naphthalene fluorescence detection Fluorescence PCR relative quantification Fluorescence concentration Fluorescence data Real-time fluorescent quantitative PCR which PCR instrument Real-time fluorescent PCR instrument and pcr Ultra-high-speed real-time fluorescence quantification pcr+instrument+machine time the time real-time pcr analysis Multiplex fluorescence quantitative PCR detection method pcr instrument during pcr Liquid phase fluorescence detection peak Fluorescence detection Fluorescent quantitative PCR analysis method Construction of PCR method labeled fluorescent detection real-time fluorescence How long is real-time fluorescent quantitative PCR Q3 fluorescent quantitative PCR fluorescent pcr pcr Fluorescent quantitative PCR analysis method Fluorescent PCR analysis fluorescent pcr phase Real-time fluorescence quantification v7 Fluorescent Molecular Detection Fluorescence Used Detectors Real-time fluorescent quantitative PCR analysis Portable real-time fluorescent quantitative PCR Fluorescence real-time quantitative PCR Fluorescence detection method of pyrene real-time pcr analysis method multiplex fluorescent quantitative PCR Fluorescence quantitative + PCR + analysis fluorescent pcr phase Pyrene fluorescence detection method real-time fluorescent quantitative pcr and pcr pcr detection method Fluorescence test before pregnancy pcr detection method pcr detection method Q5 fluorescent PCR Characteristics of Fluorescence Detection real-time fluorescence by pcr technology isothermal fluorescence detection pcr detection method Fluorescent PCR detection method Real-time fluorescent quantitative PCR peak multiplex pcr+ fluorescence pcr ultraviolet detection Several methods of real-time quantitative pcr Fluorescent PCR detection method Fluorescence PCR instrument usage method Fluorescence Detection Cleaning PCR fluorescence detection Two insect detection Fluorescent PCR cassette multiplex fluorescent PCR method multiplex real-time fluorescence quantitative pcr technology Real-time fluorescent quantitative PCR instrument q5 Pyrene fluorescence experiment Q5 real-time fluorescent quantitative PCR instrument q7 real-time fluorescent PCR instrument Multiplex fluorescent quantitative PCR detection wool fluorescent Real-time fluorescent Fluorescence PCR instrument q7 pcr instrument pcr method Methods for detecting fluorescence Optical Spectroscopy Liquid Fluorescence Test Method wool fluorescent Yingyunshi How to qualitative real-time fluorescent PCR pcr fluorescence detection concentration at optical rotation flow real-time fluorescence Relative real-time fluorescence quantification wavelength of fluorescence Fluorescein fluorescence what level does pcr detect Autofluorescence Mode Detection Features of pcr detection detection pcr method pcr+standard fluorescent r square fluorescence r squared Fluorescent timed quantitative PCR Fluorescent detection of cadmium Fluorescence PCR instrument peak shape Several detection methods of pcr Fluorescence PCR instrument method water quality testing pcr Optical rotation measurement method pcr luciferase Fluorescence and UV detectors Fluorescence detector UV detector plate time fluorescence q7 real-time fluorescent quantitative PCR instrument light detection method PCR initial fluorescence Fluorescence detection Quantitative detection by fluorescence method r2 of fluorescent quantitative pcr pcr detection method report molecular detection fluorescence Fluorescence time of autofluorescence fluorescent PCR kit fluorescent probe fluorescent pcr Real-time fluorescence semi-quantitative PCR Deuteration detection Fluorescent detection Fluorescent color detection Fluorescence online detection q6 real-time fluorescence What is the main detection of fluorescent quantitative PCR pcr re pcr pcr flow cytometry fluorescence detection Color method Fluorescent real-time quantitative PCR relative quantification fluorescent pcr iso How to use fluorescent PCR instrument polarimeter Fluorescent quantitative pcr method to detect dna fluorescent version polarimetry real-time fluorescent quantitative pcr and pcr Detection of pcr instrument temporal fluorescence measurement fluorescent pcr probe Fluorescent Time Grating Fluorescence detection five-point detection plate What is the use of pcr fluorescence Fluorescence isothermal detection Relative quantification by fluorescent PCR detection Real-time quantitative PCR analysis fluorescence capillary analysis Isothermal PCR Real-time fluorescent PCR Liquid fluorescence detection Fluorescence quantitative PCR food detection detection method of pcr instrument Effect of Optical Rotation Detection pcr non pcr PCR and real-time quantitative PCR Fluorescent Molecular Detection Fluorescence PCR detection series enzyme time pcr instrument Ordinary PCR instrument and fluorescent PCR Enzyme fluorescence detection Enzymes for pcr and fluorescent quantitative pcr What is the general fluorescence concentration when measuring fluorescence Isothermal Fluorescence Detection System Real-time quantitative PCR multiplex detection GBT35904 GB/T 35904-2018 capillary fluorescence technique fluorescence detection of urine Aquatic bacteria fluorescent PCR What are the fluorescence quantitative PCR methods? Fluorescence quantitative PCR fluorescence intensity is low miRNA fluorescence quantitative PCR U6 Fluorescence detector optical path digital PCR testing service Fluorescence spectrum response time How fluorescence detectors work Fluorescence detection limit Fluorescent fiber detection Fluorescence detection accepted Histamine fluorescence detection method Local standard fluorescence detection Fluorescein detection method Fluorescence detection method of uranium Cordycepic acid detection method Polarimeter detection Fluorescence detection of fibers Luciferase Assay Method Detection method of sodium fluorescein Fluorescence detection method of silicon in water Real-time detection of water activity tester measurement method Fluorescent antibody quality detection method Verification of specific spin detection method Two insect detection methods Fabric fluorescent agent detection Two insect detection methods Spray fluorescence detection Fluorescence and other detection methods Fluorescence detector linear calculation method Method Optimization for Optical Polarimetry Determination Cell membrane damage fluorescence detection method Firefly luciferase assay Optical rotation measurement solution preparation method Fluorescence detector method Fluorescence detector drug quality Specific rotation detection method Luciferase detection method Advantages and Disadvantages of Fluorescence Methods Advantages and Disadvantages of Fluorescence Detection Methods Cell calcium fluorescence detection method Fluorescence detection of non-woven fabrics Photosynthetic rate detection method Advantages and Disadvantages of Fluorescence Detection Methods

《GB/T 35904-2018旋毛虫实时荧光PCR检测方法》由TC181（全国动物卫生标准化技术委员会）归口，主管部门为农业农村部。

Introduction

GB/T 35904—2018 "Real-time fluorescence PCR detection method for Trichinella spiralis" is an important standard formulated by the Ministry of Agriculture of China, which aims to standardize the nucleic acid detection process of Trichinella spiralis. This article will deeply analyze the core content of the standard and interpret it with actual cases.

Standard background and technical evolution

Trichinella spiralis infection is a serious public health problem worldwide, especially in meat quarantine. Real-time fluorescence PCR technology has become the preferred method for detecting Trichinella spiralis with its high sensitivity and specificity. This standard is based on internationally leading technology to ensure the reliability of the test results.

Instruments and reagents

According to the standard requirements, the fluorescence PCR instrument is the core equipment, and it is matched with auxiliary instruments such as high-speed refrigerated centrifuge and biological safety cabinet. In terms of reagents, high-purity PCR buffer, dNTP and probes are used to ensure detection accuracy.

Comparison table of standard frameworks

Standard dimensions	GB/T 35904—2018	Differences in international standards
Detection objects	Trichinella nucleic acid	Consistent with ISO standards
Reagent requirements	High-purity biological reagents	Higher than the standards of some countries
Operation process	PCR amplification + fluorescence detection	Optimized cycling conditions

Actual application cases

In a pig farm, this standard method was used to test a batch of pigs for Trichinella spiralis. The results showed that the Ct value was 28, which was judged to be positive. This helped the farm to isolate the infected pigs in time and avoid the spread of the epidemic.

Implementation Recommendations

In order to ensure the effective implementation of the standard, it is recommended that:

Laboratories should be equipped with advanced PCR equipment
Operators should receive regular professional training
Establish a complete quality control system

More samples: QC/T 942-2021 JGJ 94-2008 GB 18306-2015

Sample only — not a preview of GB/T 35904-2018

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

GB/T 44613-2024 in English

Diagnostic techniques for bovine and caprine parainfluenza virus type 3
2024-09-29
GB/T 47130-2026 in English

Diagnostic techniques for bovine coronavirus infection
2026-01-28
GB/T 26618-2011 in English

Protocol of diagnosis for Perkinsosis
2011-06-16
GB/T 41626-2022 in English

Nomenclature and location of acupuncture points—Equus
2022-07-11
GB/T 16550-2020 in English

Diagnostic techniques for newcastle disease
2020-12-14
GB/T 32948-2016 in English

Detection of Fecal Antigen of Echinococcus granulosus by Canine Infection by Antibody Sandwich Enzyme-linked Immunosorbent Assay
2016-08-29
GB/T 43825-2024 in English

Technical specifications for canine rabies vaccination
2024-03-15