GB/T 35911-2018 in English

VALID

Real-time PCR method for detection of pseudorabies virus

Issued on:2018-02-06
Implemented on:2018-09-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 35911-2018
Document status:	VALID
Title in English:	Real-time PCR method for detection of pseudorabies virus
Title in Chinese:	伪狂犬病病毒荧光PCR检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-02-06
Implemented on:	2018-09-01
ICS Classification:	11.220-Veterinary medicine
Chinese Classification:	B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification:	GB-National Standard
Related Keywords:	real-time pcr method fluorescent pcr detection real-time fluorescent pcr technology pseudorabies virus processing sample type collection method storage conditions blood sample use
Related Topics:	super virus pcr Rabies m2 virus Used Fluorescence Detectors Viral Sequencing Methods Isothermal fluorescent PCR instrument Fluorescent PCR Multiplex Fluorescent PCR Fluorescent quantitative multiplex PCR detection Fluorescent PCR and multiplex pcr detection unit pcr+ virus fluorescence shared virus In vitro fluorescence detection Multiplex fluorescent quantitative PCR detection flow fluorescence and pcr Fluorescence detection equipment Fluorescent Material Detection counterfeit detection virus+ multiplex fluorescent quantitative pcr method Detection of fluorescent peaks flow fluorescent pcr What are the second-hand fluorescence detectors? Q5 fluorescent quantitative PCR Fluorescent PCR+ Multiplex PCR Fluorescent Quantitative PCR Fluorescent quantitative PCR multiplex PCR Fluorescent quantitative PCR analysis method multiplex pcr vs fluorescent pcr Isothermal fluorescent PCR Isothermal fluorescent quantitative PCR Fluorescent PCR detection how to use pcr Absolute Quantitative Fluorescent PCR Fluorescence detector peak On-line fluorescence detection pcr method pcr method GB/T 35911-2018 relative fluorescence quantitative pcr Q5 fluorescent PCR instrument pcr secondary pcr relative fluorescence pcr Isothermal fluorescent PCR instrument Multiplex PCR and Fluorescent Quantitative PCR Multiplex PCR and fluorescent PCR Fluorescent PCR detection In addition to virus detection liquid detection fluorescence Fluorescent German Detection by PCR, multiplex PCR and fluorescent quantitative PCR Extremely fast fluorescence quantitative PCR Fluorescent PCR detection method for pseudorabies virus Fluorescence detection method Fluorescence detection method virus fluorescence method virus standard Fluorescence Detector Premium Fluorescence detection method by flow cytometry Fluorescent Material Detection How to use the PCR instrument fluorescent pcr peak Quantitative PCR multiplex fluorescence detection Fluorescence detection led Column fluorescence detection method Pseudorabies fluorescent PCR detection gb/t+35911 absolute fluorescence quantitative pcr rna pcr method mini fluorescent pcr Q7 fluorescent quantitative PCR Absolute quantification of virus detection fluorescent virus cells chicken fluorescent pcr How to detect virus Fluorescence detector cell Viral Infection Detection Methods Fluorescence quantitative PCR peak secretory virus Fluorescence Detector Detector virus prediction New method for virus detection Q7 fluorescent PCR isothermal fluorescence detection pcr virus hiv Fluorescent Disease Detection virus device How to use pcr machine Fluorescent quantitative PCR analysis method Q6 fluorescent quantitative PCR pcr virus hiv Fluorescence detection food detection Detection of autofluorescence Epigastric virus autofluorescence detection q6 fluorescent pcr Virus detection in feed fluorescence method virus detector Abnormal disease pcr rna detection mir pcr detection Virus Light source detection method rabies virus Fluorescence detection plate Fluorescent quantitative PCR peak Fluorescence detector series Molecular fluorescence detection Fluorescence detection and UV detection virus test q7 Rabies Antibody Test Triple fluorescent quantitative PCR Fluorescence PCR relative quantification Water quality virus detection single molecule virus virus + fluorescence Multiplex fluorescence quantitative PCR detection method virus m2 Domestic water quality virus detection Fluorescence detection Virus Prion Fluorescent quantitative PCR analysis method transient virus Device virus fluorescent technology virus Q3 fluorescent quantitative PCR fluorescent pcr pcr Fluorescent quantitative PCR analysis method Fluorescent PCR analysis fluorescent pcr phase Fluorescent Molecular Detection Fluorescence Used Detectors t-virus prions Fluorescence detection method of pyrene Fluorescence quantitative + PCR + analysis fluorescent pcr phase Rabies Gold Pyrene fluorescence detection method Q5 fluorescent PCR female virus Characteristics of Fluorescence Detection Pseudorabies Pseudorabies virus isothermal fluorescence detection How to detect virus by fluorescent quantitative PCR pcr detection method Fluorescent PCR detection method Prion disease pcr ultraviolet detection ancient virus Fluorescent PCR detection method Fluorescence PCR instrument usage method PCR fluorescence detection Fluorescent PCR cassette multiplex fluorescent PCR method How does pcr detect virus Multiplex fluorescent quantitative PCR detection Virus PCR detection Fluorescence PCR instrument q7 pcr instrument pcr method Methods for detecting fluorescence pcr fluorescence detection Tissue virus PCR detection How to use fluorescent antibody to detect virus hiv virus test cytofluorescent virus what level does pcr detect Features of pcr detection detection pcr method pcr+standard Detection of r5 virus Fluorescent detection of cadmium Fluorescence PCR instrument peak shape rna virus prion Several detection methods of pcr Fluorescence PCR instrument method virus cell fluorescence water quality testing pcr How does PCR instrument detect disease x4 virus pcr luciferase Fluorescence and UV detectors light detection method PCR initial fluorescence virus way Fluorescence detection Quantitative detection by fluorescence method r2 of fluorescent quantitative pcr pcr detection method report molecular detection fluorescence pcr hiv virus test fake fluorescent pcr Pseudorabies detection Deuteration detection Fluorescent detection Fluorescent color detection Fluorescent quantitative PCR detection of virus Fluorescent virus detection What is the main detection of fluorescent quantitative PCR pcr re pcr pcr flow cytometry fluorescence detection Virus system? Virus system? fluorescent pcr iso Prion detection method Virus PCR instrument How to use fluorescent PCR instrument fluorescent version Prion method Virus Virus sampling method cold virus Detection of pcr instrument prionlow temperature component virus Fluorescence detection five-point detection plate What is the use of pcr fluorescence Fluorescence isothermal detection Relative quantification by fluorescent PCR detection Bali virus broad-spectrum virus Virus + five fluorescent Liquid fluorescence detection Fluorescence quantitative PCR food detection detection method of pcr instrument cellular virus fluorescence pcr non pcr Fluorescent Molecular Detection Fluorescence PCR detection series Virus sequencing detection viral protein virus Real-time quantitative PCR multiplex detection GBT35911 GB/T 35911-2018 Fluorescent PCR of pathogenic bacteria in aquatic products pathogenic bacteria fluorescent pcr Rabies cases What are the fluorescence quantitative PCR methods? Fluorescence quantitative PCR fluorescence intensity is low simply P virus miRNA fluorescence quantitative PCR U6 Fluorescence detector optical path digital PCR testing service Virus cryopreservation methods Comparison of viral prions Viralin discontinued ev70 virus The difference between viruses, viroids, and prions Prion detection and sampling methods Fluorescence detection limit retro virus The difference between prions and viruses Rabies Antibody Test Fluorescent fiber detection Pseudovirus purification method Fluorescence detection accepted Lentivirus cryopreservation method Histamine fluorescence detection method Virus killing behavior detection method Methods to study virus release Virus titer determination method Principles of virus purification methods Local standard fluorescence detection Fluorescein detection method Fluorescence detection method of uranium Antiviral drug testing methods How to store viruses Virus infection testing methods Fluorescence detection of fibers Luciferase Assay Method Diagram of syphilis detection methods How to detect viral infection Advantages and Disadvantages of Virus Detection Methods Ketosis detection method Virus checking methods Plant virus disease detection methods Residual virus detection limit Methods for detecting pseudoviruses using transmission electron microscopy Spectral disease detection method Fabric fluorescent agent detection Virus suspension concentration method Fluorescence and other detection methods Fluorescence detector linear calculation method Cell membrane damage fluorescence detection method Fluorescence detector method Luciferase detection method Advantages and Disadvantages of Fluorescence Detection Methods Cell calcium fluorescence detection method Fluorescence detection of non-woven fabrics Advantages and Disadvantages of Fluorescence Detection Methods

《GB/T 35911-2018伪狂犬病病毒荧光PCR检测方法》由TC181（全国动物卫生标准化技术委员会）归口，主管部门为农业农村部。

Introduction

Interpretation of GB/T 35911—2018 National Standard of the People's Republic of China

Standard Overview

This standard specifies the operating method of fluorescent PCR detection of pseudorabies virus, which is applicable to the qualitative and quantitative analysis of nucleic acids. This method uses real-time fluorescent PCR technology combined with specific primers and probes to achieve rapid and sensitive detection of PRV.

Instruments and Equipment

Instrument Name	Model Requirements	Functional Description
Fluorescence PCR Detector	Real-time monitoring of fluorescence signal	Core equipment for amplification curve analysis and Ct value calculation
High-speed desktop refrigerated centrifuge	Temperature controllable to 4℃, above 12000r/min	Used for sample processing and reagent separation

Reagents and Consumables

Main reagent requirements

DNAzol: commercial DNA extraction reagent, stored at 2~8℃
Anhydrous ethanol: pre-cooled at -20℃
Taq enzyme and buffer: concentration 5U/μL, containing 15mmol/L Mg²+

Sample collection and processing

Sample type	Collection method	Storage conditions
Blood sample	Use a sterile syringe and add EDTA anticoagulant	Store at 2-8℃ for no more than 24 hours
Tissue sample	Take 1g of thawed tissue and prepare homogenate	Store for a long time below -70℃

Fluorescence PCR operation procedure

DNA extraction steps

Add 800μL DNAzol to a 1.5mL centrifuge tube
Add 200μL sample and mix by inversion
Centrifuge at 10,000r/min for 10 minutes at 4℃ or room temperature

Result judgment and quality control

Threshold setting: Adjust based on instrument noise to ensure that there is no amplification curve in the negative control. Positive control requirements: Ct value of FAM and HEX channels ≤30, Ct value of ROX channel ≤36.

Implementation suggestions

The laboratory should have low-temperature storage equipment (below -70°C)
Operators should be professionally trained and familiar with PCR technology
Regularly calibrate the instrument to ensure detection accuracy
Establish a quality control system, including positive/negative controls and blank tests

More samples: JC/T 2453-2018 QC/T 942-2021 YD/T 3934-2021

Sample only — not a preview of GB/T 35911-2018

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

GB/T 44613-2024 in English

Diagnostic techniques for bovine and caprine parainfluenza virus type 3
2024-09-29
GB/T 26618-2011 in English

Protocol of diagnosis for Perkinsosis
2011-06-16
GB/T 47130-2026 in English

Diagnostic techniques for bovine coronavirus infection
2026-01-28
GB/T 16550-2020 in English

Diagnostic techniques for newcastle disease
2020-12-14
GB/T 39602-2020 in English

Diagnostic techniques for Lumpy skin disease
2020-12-14
GB/T 17494-2023 in English

Diagnostic techniques for equine infectious anemia
2023-09-07
GB/T 34720-2017 in English

Diagnostic techniques for contagious caprine pleuropneumoni
2017-11-01
GB/T 18637-2018 in English

Technical specifications for diagnosis of bovine viral diarrhea/mucosal disease
2018-09-17
GB/T 32948-2016 in English

Detection of Fecal Antigen of Echinococcus granulosus by Canine Infection by Antibody Sandwich Enzyme-linked Immunosorbent Assay
2016-08-29
GB/T 22332-2025 in English

Diagnostic techniques for duck virus enteritis
2025-08-29