GB/T 36778-2018 in English
VALIDDetection and identification of Oat mosaic virus
- Issued on:2018-09-17
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$185.00
《GB/T 36778-2018燕麦花叶病毒检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
1. Standard Overview
GB/T 36778-2018 "Oat Mosaic Virus Quarantine and Identification Method" is a national standard of the People's Republic of China, which is used to regulate the detection and identification of oat mosaic virus. This standard was proposed and coordinated by the National Technical Committee for Plant Quarantine and Standardization, and the main drafting units include Ningbo Entry-Exit Inspection and Quarantine Bureau, Zhejiang Academy of Agricultural Sciences, etc.
2. Basic information of oat mosaic virus
| Classification status | Scientific name | Synonym | Abbreviation |
|---|---|---|---|
| Potyviridae, Hordeovirus genus | Oatmosaicvirus | Soil-borne oat mosaic virus | OMV |
3. Principle of detection method
This standard adopts the following three main detection techniques:
- DAS-ELISA: Double antibody sandwich enzyme-linked immunosorbent assay, used for rapid quantitative detection.
- RT-PCR: Reverse transcription polymerase chain reaction, suitable for amplification and identification of viral nucleic acids.
- IEM: Immunoelectron microscopy, which is determined by observing the morphological characteristics of viral particles.
4. Preparation of test samples
For seed testing, at least 1000 seeds should be picked and divided into several portions (25-100 seeds per portion), and then soaked in sterile distilled water for about 12-24 hours before testing. For plant samples, leaves are selected according to the symptoms.
5. Testing and identification steps
DAS-ELISA test process
- The coated antibody is diluted and added to the wells of the enzyme-linked plate, and incubated at 37℃ for 2 hours.
- After washing, add the prepared sample and incubate at 37℃ for 2 hours.
- Add enzyme-labeled antibody and incubate at 37℃ for 1 hour.
- Add substrate for color development, read OD value at 405nm, and judge the result.
6. Result determination
If the sample is positive by DAS-ELISA test and RT-PCR or IEM test is also positive, it is determined that oat mosaic virus is detected. RT-PCR amplification results can also be determined to be positive when the consistency with the known sequence is not less than 80%.
7. Implementation suggestions
In actual application, it is recommended that:
- Strengthen the construction of the quarantine team to ensure that the testing personnel are familiar with the standard requirements.
- Establish a complete quality control system to ensure the accuracy and reliability of the test results.
- Promote regional cooperation and share oat mosaic virus monitoring data.
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