GB/T 36852-2018 in English
VALIDDetection and identification of Erwinia pyrifoliae
- Issued on:2018-09-17
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$204.00
《GB/T 36852-2018亚洲梨火疫病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
GB/T 36852—2018 Standard Overview
This standard was proposed and coordinated by the National Technical Committee for Plant Quarantine Standardization. The main drafting units include China Inspection and Quarantine Scientific Research Institute, Nanjing Agricultural University, etc.
Basic information on Asian fire blight
| Classification status | Route of transmission | Host range |
|---|---|---|
| Kingdom of Bacteria, Phylum Proteobacteria, Class Y-Proteobacteria, Order Enterobacteriales, Family Enterobacteriaceae, Genus Erwinia | Infected pear propagation materials, insects, wind, rain and contaminated tools | Asian pear (Pyrus pyrifoliae Nakai) and some varieties of Western pear |
Principles of quarantine and identification methods
Based on immunological detection, molecular biological detection and isolation and culture technology.
- PCR gel electrophoresis detector: for amplification product analysis
- Real-time fluorescence PCR instrument: for quantitative detection
- Biolog identification system: automatic identification of microorganisms
Implementation suggestions
1. The laboratory should be equipped with all instruments and equipment required by the standard, such as clean bench, PCR instrument, etc.
2. During sample collection and processing, the aseptic operation procedures must be strictly followed to avoid cross contamination.
3. For molecular biology detection, it is recommended to give priority to the real-time fluorescence PCR method to ensure detection sensitivity and specificity.
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