GB/T 37628-2019 in English

GB/T 37628-2019 Standard Overview and Scope of Application

This standard specifies the HPLC determination method of astragaloside IV, paeoniflorin, forsythiaside and forsythiaside A in cosmetics, and is applicable to the detection of aqueous solutions, emulsions and creams. Its main features include:

• Clear detection limit and quantitative limit
• Evaporative light scattering detector and ultraviolet detector
• Standard curve establishment and external standard method quantification

Background of Standard Development

With the widespread use of plant extracts in cosmetics, ensuring their safety and effectiveness has become an important issue. This standard aims to provide a scientific basis for the quantification of relevant ingredients and meet the national demand for cosmetics quality supervision.

Detection principle and method

This standard adopts high performance liquid chromatography (HPLC) and is implemented by the following steps:

1. Extraction:After dissolving in hot water, extract with n-butanol.
2. Purification:Purification by C18 solid phase extraction column.
3. Separation:Use ODS-C18 chromatographic column.
4. Detection:Evaporative light scattering or UV detector.

Result Analysis and Precision

The standard stipulates strict precision requirements to ensure data reliability.

Implementation Recommendations

To ensure the accuracy of the test, it is recommended that:

• Operators must undergo professional training.
• Equipment must be calibrated and maintained regularly.
• Strictly follow standard procedures.