GB/T 38164-2019 in English

VALID

Identification of animal ingredient from common livestock and poultry—Real-time PCR

Issued on:2019-10-18
Implemented on:2019-10-18
File Format:PDF
Delivery:Within 1 day

Price(USD)： $128.00
$125.00

Standard No:	GB/T 38164-2019
Document status:	VALID
Title in English:	Identification of animal ingredient from common livestock and poultry—Real-time PCR
Title in Chinese:	常见畜禽动物源性成分检测方法实时荧光PCR法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Within 1 day
Issued on:	2019-10-18
Implemented on:	2019-10-18
Professional Classification:	GB-National Standard
Related Keywords:	real-time fluorescence pcr real-time pcr real-time fluorescence pcr instrument real-time fluorescence pcr detection method poultry real-time pcr introduction in-depth interpretation real-time fluorescence pcr detection amplification
Related Topics:	pcr animal origin Composition testing Visible light on time Fluorescent PCR+ Fluorescent quantitative PCR analysis method Isothermal fluorescent PCR Multiplex real-time PCR analysis method pcr method pcr method relative fluorescence pcr fluorescent component Multiplex PCR and fluorescent PCR Multiplex PCR real-time fluorescence quantitative PCR Real-time fluorescence quantitative PCR analysis fluorescent signal Fluorescence detection method by flow cytometry fluorescent pcr peak Multiplex fluorescent PCR Real-time fluorescent PCR Column fluorescence detection method rna pcr method When detecting fluorescence spectra? Composition detection method pcr amplification upstream primer Common pcr instrument abnormal pcr streaks Q7 fluorescent PCR animal origin ingredients animal source Composition detection method Common Bromination Methods Fluorescent quantitative PCR analysis method Analytical method for real-time fluorescence quantification Common Methods of Separation q6 fluorescent pcr Animal composition ratio test Methods for detection of ingredients of animal origin animal source pcr animal derived ingredients pcr Light source detection method amplified pcr ingredients of animal origin Fluorescence data pcr instrument common Fluorescent Molecular Components Fluorescent quantitative PCR analysis method Non-Animal Origin Ingredients How to test for ingredients of animal origin Fluorescent quantitative PCR analysis method Animal Origin Component Testing Real-time fluorescent quantitative PCR analysis Fluorescence real-time quantitative PCR real-time pcr analysis method real-time fluorescent quantitative pcr and pcr real-time PCR source ingredient Avian Q5 fluorescent PCR Common Analysis Methods Characteristics of Fluorescence Detection Fluorescence PCR instrument usage method How to qualitative real-time fluorescent PCR Several common pcr flow real-time fluorescence Common Quantitative Analysis Methods pcr amplification detection pcr fluorescence method unconventional pcr pcr+ method Fluorescence PCR instrument method plate time fluorescence Secondary PCR common method pcr common Composition Standard fluorescent pcr q6 real-time fluorescence pcr flow cytometry fluorescence detection In vivo detection of fluorescent animals In vivo detection of fluorescent animals fluorescent pcr iso pcr components common food ingredients common pcr real-time fluorescent quantitative pcr and pcr Method for detection of substance composition Fluorescence is usually measured at pcr common Isothermal PCR Real-time fluorescent PCR Methods for detection of ingredients of animal origin Fluorescence PCR detection series pcr fluorescence method GBT38164 GB/T 38164-2019 Common substance separation method names What are the fluorescence quantitative PCR methods? Characteristics of fluorescence generation method Road mobile source VOCs detection method Bioactive ingredient detection methods Histamine fluorescence detection method Common analgesic experimental methods Common instrumental analysis methods Copper rod composition detection method spectrum Common pathogen vaccination methods Common energy spectrum analysis methods Common testing methods for paraffin Method for detecting endogenous fluorescence using microplate reader Fluorescein detection method Common molecular hybridization methods are divided into Commonly used ingredient detection methods Commonly used quantitative detection methods EU animal origin testing What are the commonly used biomass detection methods? Common methods of antigen testing Common electrochemical testing methods Common pharmaceutical quantitative analysis methods Homology detection methodology Common methods for detecting amino acids Fluorescence and other detection methods Fluorescence detector linear calculation method Fluorescence detector method Common laboratory staining methods Testing methods for common indicators of water quality Film forming test method Common staining methods in laboratories gbt38164-2019 Advantages of Fluorescence Imaging Methods Fluorescence analysis method for animal live imaging Common polarized light detection methods Common quantitative and qualitative analysis methods Common electrochemical methods Common biological sample extraction and testing methods Common methods of expressing enzyme activity gb/t38164

《GB/T 38164-2019常见畜禽动物源性成分检测方法实时荧光PCR法》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

In-depth interpretation of the National Standard of the People's Republic of China GB/T 38164—2019 Real-time fluorescence PCR detection method for animal-derived ingredients in livestock and poultry

I. Scope of the standard and applicable fields

This standard applies to:

Detection of components of cattle, yaks, buffaloes and other species in meat and processed products
Identification of animal-derived ingredients such as sheep, goats, pigs, etc. in dairy products
Qualitative analysis of alien species (such as camels and red deer) in animal feed
Quality control of dog, cat, rabbit and other ingredients in pet food

II. Comparison table of standard frameworks

Standard dimensions	GB/T 38164—2019	International similar standards
Detection method	TaQman probe real-time fluorescence PCR	Real-time PCR (American ASTM E2876 standard)
Number of target species	Covering 30 common livestock and poultry animals	About 15 basic species (EU EN 16472 standard)
Sensitivity	LOD 1%Mass fraction	LOD 0.5% (U.S. Department of Agriculture standard)

III. Core technology analysis and application cases

1. Principle of real-time fluorescence PCR detection

Amplification is performed through species-specific primers and probes, and the entire PCR process is monitored using fluorescence signals. Key indicators: Ct value (Cycle Threshold) is used to determine whether the target component exists.

2. Reagent and instrument requirements

Real-time fluorescence PCR instrument: Ensure that the temperature control accuracy is ±0.1℃, and the fluorescence collection sensitivity meets the standard requirements.
Nucleic acid purification reagents (such as CTAB extraction buffer) must reach the first-class water purity.

IV. Implementation recommendations

Sample pretreatment:Solid samples are washed with 70% ethanol and double distilled water, and semi-solid/liquid samples need to be fully mixed before packaging.
DNA extraction:It is recommended to use the GB/T 35918 standard method or equivalent kit to ensure that the DNA concentration is within the range of 5-50 ng/μL.
Quality control:Positive control, negative control and blank control should be set for each batch of testing, and the Ct value range should be strictly monitored (reference gene Ct<30.0, blank control Ct≥40.0).

More samples: JC/T 2453-2018 NB/T 47013.2-2015 DL/T 5154-2012

Sample only — not a preview of GB/T 38164-2019

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