GB/T 38517-2020 in English
VALIDParticulate—Bioaerosols sampling and analysis—General principles
- Issued on:2020-03-06
- Implemented on:2020-06-01
- File Format:PDF
- Delivery:Within 1 day
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《GB/T 38517-2020颗粒 生物气溶胶采样和分析 通则》由TC168(全国颗粒表征与分检及筛网标准化技术委员会)归口,TC168SC1(全国颗粒表征与分检及筛网标准化技术委员会颗粒分会)执行,主管部门为国家标准化管理委员会。
Introduction
Interpretation of the core content of the standard
This standard systematically specifies the full-process technical requirements for the sampling of biological aerosols in ambient air, with the key points including:
1. Sampling method system- 7 basic sampling principles (impact/impact/membrane/centrifugal, etc.)
- Differentiated sampling schemes for 4 biological components (bacteria/virus/fungus/toxins)
Comparison of key technologies
| Sampling type | Applicable microorganisms | Maximum sampling time | Influence on survival rate |
|---|---|---|---|
| Impact type | Bacteria, fungi | 15min | Medium |
| Impact type | Virus, toxin | 30min | Low |
| Membrane filter type | All types | 10min | High |
Analysis of implementation points
- Choose Slit impact sampler with Sabouraud culture medium
- Sampling points are arranged according to GB50591 standard
- Sampling time is controlled between 8-15 minutes
- Colony count is performed after 7 days of incubation at 28℃
Safety protection requirements
Corresponding measures should be taken according to different biosafety levels:
- BSL-1 level: basic personal protective equipment
- BSL-2 level: N95 mask + goggles
- BSL-3 level: positive pressure protective clothing + two-person operation

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