GB/T 40172-2021 in English

VALID

General guidance on detection methods of mammalian cell cross-contamination

Issued on:2021-05-21
Implemented on:2021-12-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $250.00
$243.00

Standard No:	GB/T 40172-2021
Document status:	VALID
Title in English:	General guidance on detection methods of mammalian cell cross-contamination
Title in Chinese:	哺乳动物细胞交叉污染检测方法通用指南
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2021-05-21
Implemented on:	2021-12-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	A40-Basic Subject in general
Professional Classification:	GB-National Standard
Related Keywords:	detection methods detection methods mammalian cell cross-contamination detection detection methods mammalian cell cross-contamination introduction interpretation key detection nodes
Related Topics:	animal cell cross cell alpha cells multicellular animal Mesozoic mammals Mammals Mesozoic cell detection method cellular contaminants cell english In situ detection of cells long-term cells cell contamination cell exchange mammalian cells Benzene pollutant detection stained cells Guidelines for Assay Methods In situ detection of cells cell species Apoptosis detection cell marker detection cells Primary cells Cell line detection method Law cells cell properties cell contamination Cell Detection Analysis primary cells if cell mammalian momentary mammalian exosomes mammalian instant animal cell mammalian cell Pollution detection During cell detection labeled cells cell standard Pollution source detection method cell apoptosis detection homogeneous cells Pollution detection Cell Detection System Detection of cell generation Cell passage for detection mixed cell mammalian cells nocturnal mammals two kinds of cells Pre mammalian 8 cells Apoptosis detection Mammalian serum method cell version cell detection method growing cells Cell + Bacteria kind of cells Detection cell analysis Detection method of apoptosis cell version cell surface nocturnal mammals nocturnal mammal Mammals of the same sex In situ detection in cells pollution law in situ detection of cells Cross-contamination cell detection cell Methods for detecting apoptosis Apoptosis detection method Dye-based cell detection Cytometry Methods of Cell Analysis in situ detection of cells flow assay in situ cell detection Triassic mammals Apoptosis detection method How cells detect Triassic mammals Detection of Contaminants in Cell Culture time animal cells mammal + male cross detection Final Cell Detection Detection cell method animal cell method pollution law cell surface Parietal Cell Apoptosis Assay single mammalian parent Cell level detection adherent growth adherent growth "cell" animal cell tank Apoptotic detection method How to detect apoptosis Dairy Contaminants Cell Dye Detection Cell double staining cell english Animal immune cell detection method cell detection test Detection of apoptosis mammal relative Detection method of apoptotic cells Three-cell detection method single mammalian parent mammal relative animal double stained cells double stained cells Cell Growth Assay cell spectrum acellular animal How long cells are not detected How to detect cell function homogeneous cells How to Double Stain Cells Apoptosis and cell detection Apoptosis detection method Apoptosis, detection method pre-mammal Cellular Level and Animals nocturnal mammal cell if test cells animal cell coat Detection of cell growth Apoptosis detection method Three staining of cells Apoptosis detection cell growth cell Apoptosis detection method cell + version transient mammalian cells cell marker 100 million years ago mammals How to measure animal cells Cell series detection Apoptosis detection method as opposed to mammals /object cell mammalian mass ancient mammals stained cells Detection method of early cell apoptosis How to detect cells Apoptosis detection of cells flow staining cell staining GBT40172 GB/T 40172-2021 Mammalian two-hybrid applications lipo2000 transfected cell lines caco-2 cell contamination transfection of jurkat cells Methods to detect changes in cell membrane permeability Pollution index detector Materials live and dead cell staining methods Detection methods for drugs acting on organelles Stem cell differentiation potential detection method Quantitative methods for detecting specific cells Methods for breaking animal cell membranes Methods to detect cell viability Cell biology detection methods Which method is used to stain cultured muscle cells? How to measure the action potential of a cell Emulsification detection method Cell crystal violet staining method Thallium contaminant detection method Commonly used cell differentiation methods Nuclear contamination detection methods Methods for detecting intracellular drug concentrations Cell membrane integrity detection method What are the detection methods for gas pollutants? Cell membrane phospholipid detection method Method for detecting pyruvate in cells Animal cell fusion methods Methods to detect cell membrane permeability Immune cell marker detection methods Methods for detecting mycoplasma contamination in cells Syncytium detection method Methods for isolating animal cells Neutrophil staining method Animal cell membrane disruption method Mechanical methods for dispersing animal cells Various pollutant detection methods Cell biochemical staining methods Methods for detecting animal and vegetable oils in sewage Specific methods for detecting chromium as a pollutant Cell proliferation assay Methods to promote animal cell fusion Cell cycle checkpoint detection methods A commonly used method to detect cytotoxic effects is Cat cross-matching method Suspension cell viability detection method Leukocyte esterase detection method Mammalian cell disruption methods Mammalian cell culture media industry Detection method for carry-over contamination rate Methods to avoid cell contamination How to stain cells with trypan blue New pollutant detection methods Mammalian red blood cell hemolysis rate test method Stem cell detection methods Method for measuring cytosolic lactate levels Methods for introducing plasmids into animal cells Methods for mechanically obtaining single animal cells Animal cell fusion methods Animal cell lysis method Methods to detect cell proliferation Methods to reduce contamination in cell culture Stem cell particle size detection method Method for detecting intracellular lactate concentration Osteoclast detection method Detection methods for inorganic gaseous pollutants The most accurate method for detecting cell viability Cell penetration detection method Cell viability detection method Cell membrane integrity detection method Cell fusion refers to artificial methods Cell bank assay and method introduction Advanced methodology of transfection cell method Cell fusion detection method Cell viability detection methods and principles Methods to detect cell surface antigens Cell membrane damage fluorescence detection method What method is used to detect cell activation ability? Methods for detecting amino acids in cells New method for detecting stem cell differentiation Detection methods for cytoskeletal rearrangements Commonly used detection methods for cells Cell membrane permeability detection method Cellular iron staining methods Methods for dispersing animal cells Nuclear pollution detection water quality detection method Cell viability detection method trypan blue Cell transfection procedures What are the methods for detecting air pollutants? How to detect cell membrane permeability Application of single-cell sequencing methods Cell extract preparation method Staining method cells Common cell staining methods Intracellular amino acid detection method Cell breaking method Nucleus staining method Animal cell fixation methods Cell calcium fluorescence detection method Plant cell fusion detection method Cell invasion assay staining method Two methods of cell communication Common animal cell fixation methods Detection methods for cell culture contamination Commonly used methods to detect cytotoxic effects are Cell chromosome extraction method Plant cell gap measurement method Red blood cell staining method Methods for detecting stem cell differentiation potential Large-scale culture method of mammalian cells Methods for detecting amino acids entering cells Cell transfection method There are several cell staining methods. gbt40172-2021 Animal cell protoplast method Mammalian cell transfection methods Methods for testing food for bacterial contamination Cellular methylation level detection method Method for detecting the degree of cell rupture Image of new pollutant detection method

《GB/T 40172-2021哺乳动物细胞交叉污染检测方法通用指南》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

Interpretation of the core content of the standard

GB/T40172-2021 is my country's first special standard for mammalian cell cross-contamination detection. It systematically standardizes the full chain of technical requirements from detection principles to implementation process. This standard places special emphasis on ensuring the purity of cell culture through a multi-dimensional detection strategy. The main technical highlights include:

Establishing a two-level detection system between species and within species
Clarifying the applicable scenarios of 9 types of detection methods
Specifying the 9 core loci that must be included in STR typing
Proposing key detection nodes in the early culture stage (first week)

Comparative analysis of detection methods

Detection methods	Applicable scenarios	Sensitivity	Key technical indicators
STR genotyping	Intra-species contamination	High (1%)	9 core loci including D13S317 must be tested
PCR detection method	Inter-species contamination	High (0.1%)	CYTB/CO1 gene difference analysis
Isozyme spectrum	Inter-species contamination	Low (10%)	Four enzyme spectra of G6PD/LDH/MD/NP

Key points for the implementation of key technologies

STR typing detection specifications

When identifying human cells, a composite amplification system containing 9 STR loci including D13S317, TH01, D5S818 is required, and data analysis is performed according to the ATCC ASN ANSI 0002-2011 standard. A typical example is the identification of HeLa cells, which needs to match the standard STR profile provided by ATCC.

Identification of interspecies contamination

When using chromosome karyotype analysis, please note:

Human cells are diploid (2n=46)
Mouse cells have 40 chromosomes (2n=40)
Aneuploidy needs to be confirmed in combination with STR typing

Laboratory quality management

Key control links

Staff training: Need to pass the dual-module assessment of cell culture technology and molecular detection technology
Equipment verification: PCR instrument needs to be calibrated with temperature gradient regularly
Control setting: Each batch of testing must include positive/negative controls of known origin

Test cycle recommendation

Cell type	Test frequency
Primary cells	Culture day 3 + before freezing
Passaged cell lines	Every 3 months or every 10 generations

More samples: JGJ 94-2008 YD/T 3934-2021 JC/T 2453-2018

Sample only — not a preview of GB/T 40172-2021

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

GB/T 27990-2011 in English

Fundamental terms for biochips
2011-12-30
GB/T 33681.1-2017 in English

Methods to prepare samples for high-throughput gene sequencing—Part 1：Preparing samples of animal tissues
2017-05-12
GB/T 47142-2026 in English

Determination of molecular weight of polysaccharides—Size exclusion chromatography with multi-angler laser light scattering method
2026-01-28
GB/T 34776-2017 in English

T4 DNA ligase-Detection methods of activity and impurities of enzyme
2017-11-01
GB/Z 176-2026 in English

Biotechnology—Bioprocessing—General requirements and considerations for equipment systems used in the manufacturing of cells for therapeutic use
2026-04-30