GB/T 40642-2021 in English

Technical background of the standard

With the development of the functional food industry, 1-deoxynojirimycin (DNJ) in mulberry leaf extract, which has hypoglycemic activity, has become a key detection indicator. This standard fills the gap in domestic DNJ detection methods and adopts FMOC-Cl derivatization-HPLC coupling technology to solve the pain point of insufficient sensitivity of traditional methods.

Core Detection Principle

Key Operation Specifications

Quality Assurance System

• Precision control: 5 repeated determinations RSD≤5%
• Linear range: 0.01-0.10mg/mL (r²≥0.999)
• Recovery requirement: 90-110% (spiking concentration 0.5-1.5 times)

Implementation suggestions

Laboratories should pay special attention to the following:
1. FMOC-Cl solution must be prepared and used immediately
2. After derivatization, the sample should be protected from light and tested within 4 hours
3. The chromatographic column is recommended to use C18(5μm,250×4.6mm) specifications