GB/T 6437-2018 in English

VALID

Determination of phosphorus in feeds—Spectrophotometry

Issued on:2018-09-17
Implemented on:2019-04-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $100.00
$97.00

Standard No:	GB/T 6437-2018
Document status:	VALID
Title in English:	Determination of phosphorus in feeds—Spectrophotometry
Title in Chinese:	饲料中总磷的测定分光光度法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-09-17
Implemented on:	2019-04-01
Superseding:	GB/T 6437-2002 Determination of phosphorus in feed--Spectphotometry
ICS Classification:	65.120-Animal feeding stuffs
Chinese Classification:	B46-Livestock and poultry feed and additive
Professional Classification:	GB-National Standard
Related Keywords:	phosphorus determination method clarify phosphorus standard stock solution modify total phosphorus total phosphorus content standard determination principle
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《GB/T 6437-2018饲料中总磷的测定分光光度法》由TC76（全国饲料工业标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

Interpretation of the standard for determination of total phosphorus content in feed

1. Background and revision of the standard

GB/T 6437—2018 replaces the old version of GB/T 6437—2002. The main revisions include:

Delete the restriction of "except phosphate" in the scope
Add detection limit and quantification limit
Revise the statement of the principle of phosphorus determination method
Clarify the shelf life of phosphorus standard stock solution
Modify the sampling and sample preparation methods
Delete the restriction on monocalcium phosphate in the dry ashing method
Adjust the range of the standard curve

2. Scope of application of the standard

This standard applies to the determination of phosphorus in feed raw materials and products. It is recommended to use>Spectrophotometer was used for quantitative analysis.

Item	Old version (GB/T 6437—2002)	New version (GB/T 6437—2018)
Range limit	Except phosphate	No limit
Detection limit	-	20 mg/kg
Standard curve range	0-16 μg/mL	0-15 μg/mL

3. Standard determination principle and method

The total phosphorus in the sample reacts with ammonium vanadium molybdate under acidic conditions to form a yellow vanadium molybdenum yellow complex, whose absorbance is proportional to the phosphorus concentration. Use a UV-Visible spectrophotometer to determine at a wavelength of 400 nm.

Case Analysis: Wet Digestion Operation Procedure

Weigh 0.5-5 g of sample
Add nitric acid (30 mL) and heat to boil until yellow smoke disappears
Add perchloric acid (10 mL) and continue heating until white smoke appears
After cooling, dilute with water and transfer to a volumetric flask
Make up to volume and shake well for testing

4. Implementation Suggestions and Precautions

To ensure the accuracy of the measurement:

Strictly control the purity and dosage of reagents
Use qualified instruments and equipment (such as high temperature furnace and spectrophotometer)
Regularly calibrate the standard curve
Avoid cross contamination

More samples: QC/T 942-2021 GB 50021-2001(2009) DL/T 368-2010

Sample only — not a preview of GB/T 6437-2018

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